Detailed information of TA system TA020012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/-
• Location: 247297..247838
• Replicon: chromosome
• Accession: CP009789
• Organism: Escherichia coli K-12 strain ER3413

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: ER3413_229
• Protein ID: AIZ52031.1
• Coordinates: 247297..247575 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: ER3413_230
• Protein ID: AIZ52042.1
• Coordinates: 247578..247838 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER3413_226	244879..245457	+	579	AIZ52000.1	D-sedoheptulose 7-phosphate isomerase	-
ER3413_228	245663..246430	+	768	AIZ52020.1	putative amidotransfease	-
ER3413_227	246401..247141	-	741	AIZ52009.1	hypothetical protein	-
ER3413_229	247297..247575	-	279	AIZ52031.1	translation inhibitor toxin of toxin-antitoxin pair YafQ/DinJ	Toxin
ER3413_230	247578..247838	-	261	AIZ52042.1	antitoxin of YafQ-DinJ toxin-antitoxin system	Antitoxin
ER3413_231	248048..248797	+	750	AIZ52052.1	putative lipoprotein and C40 family peptidase	-
ER3413_232	248973..249470	+	498	AIZ52061.1	RAYT REP element-mobilizing transposase; TnpA(REP)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10846.62 Da
• Isoelectric Point: 9.9941

>T20012 AIZ52031.1 CP009789:c247575-247297 [Escherichia coli K-12]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T20012 CP009789:c247575-247297 [Escherichia coli K-12]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9405.80 Da
• Isoelectric Point: 5.0758

>AT20012 AIZ52042.1 CP009789:c247838-247578 [Escherichia coli K-12]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT20012 CP009789:c247838-247578 [Escherichia coli K-12]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA