Detailed information of TA system TA200095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3813626..3814320
• Replicon: chromosome
• Accession: NZ_CP073718
• Organism: Escherichia coli strain CX08

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: J6O57_RS19010
• Protein ID: WP_001263493.1
• Coordinates: 3813626..3814024 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: J6O57_RS19015
• Protein ID: WP_001399937.1
• Coordinates: 3814027..3814320 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O57_RS18980 (3808689)	3808689..3809147	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
J6O57_RS18985 (3809408)	3809408..3810865	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
J6O57_RS18990 (3810922)	3810922..3811443	-	522	Protein_3717	peptide chain release factor H	-
J6O57_RS18995 (3811439)	3811439..3811645	-	207	Protein_3718	RtcB family protein	-
J6O57_RS19000 (3811927)	3811927..3812907	-	981	WP_049109356.1	IS5-like element IS5 family transposase	-
J6O57_RS19005 (3813164)	3813164..3813616	-	453	WP_072146325.1	GNAT family N-acetyltransferase	-
J6O57_RS19010 (3813626)	3813626..3814024	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
J6O57_RS19015 (3814027)	3814027..3814320	-	294	WP_001399937.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
J6O57_RS19020 (3814372)	3814372..3815427	-	1056	WP_001226161.1	DNA polymerase IV	-
J6O57_RS19025 (3815498)	3815498..3816283	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
J6O57_RS19030 (3816255)	3816255..3817967	+	1713	Protein_3725	flagellar biosynthesis protein FlhA	-
J6O57_RS19035 (3818285)	3818285..3818782	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR / vgrG/tssI / hcp2/tssD2 / tssA / hcp1/tssD1	3778703..3844066	65363
	-	flank	IS/Tn	-	-	3811927..3812907	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T200095 WP_001263493.1 NZ_CP073718:c3814024-3813626 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T200095 NZ_CP097709:c5012399-5011644 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAACTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11267.78 Da
• Isoelectric Point: 5.2170

>AT200095 WP_001399937.1 NZ_CP073718:c3814320-3814027 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARFEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT200095 NZ_CP097709:c5012901-5012533 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Structures

Toxin

• AlphaFold DB: S1EXB8