Detailed information of TA system TA200076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 600635..601434
• Replicon: chromosome
• Accession: NZ_CP073718
• Organism: Escherichia coli strain CX08

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: J6O57_RS02980
• Protein ID: WP_000347267.1
• Coordinates: 600635..601099 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: J6O57_RS02985
• Protein ID: WP_001302819.1
• Coordinates: 601099..601434 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O57_RS02950 (596179)	596179..596958	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
J6O57_RS02955 (596969)	596969..597143	-	175	Protein_579	PTS sugar transporter subunit IIB	-
J6O57_RS02960 (597172)	597172..597869	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
J6O57_RS02965 (597884)	597884..598219	-	336	Protein_581	PTS N-acetylgalactosamine transporter subunit IIB	-
J6O57_RS02970 (598242)	598242..599522	-	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
J6O57_RS02975 (599771)	599771..600580	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
J6O57_RS02980 (600635)	600635..601099	-	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
J6O57_RS02985 (601099)	601099..601434	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
J6O57_RS02990 (601583)	601583..603154	-	1572	WP_057107993.1	galactarate dehydratase	-
J6O57_RS02995 (603529)	603529..604863	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
J6O57_RS03000 (604879)	604879..605649	+	771	WP_001559981.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T200076 WP_000347267.1 NZ_CP073718:c601099-600635 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T200076 NZ_CP097698:8716-9021 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT200076 WP_001302819.1 NZ_CP073718:c601434-601099 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT200076 NZ_CP097698:8496-8714 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4