Detailed information of TA system TA200022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2748944..2749569
• Replicon: chromosome
• Accession: NZ_CP073677
• Organism: Cupriavidus sp. KK10

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: KB879_RS13040
• Protein ID: WP_153953472.1
• Coordinates: 2748944..2749222 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KB879_RS13045
• Protein ID: WP_035815276.1
• Coordinates: 2749234..2749569 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB879_RS13040 (KB879_13040)	2748944..2749222	+	279	WP_153953472.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KB879_RS13045 (KB879_13045)	2749234..2749569	+	336	WP_035815276.1	HigA family addiction module antitoxin	Antitoxin
KB879_RS13050 (KB879_13050)	2749619..2750989	-	1371	WP_013953222.1	serine hydrolase	-
KB879_RS13055 (KB879_13055)	2751036..2751515	-	480	WP_013953223.1	MarR family transcriptional regulator	-
KB879_RS13060 (KB879_13060)	2751694..2752290	-	597	WP_013953224.1	GTP cyclohydrolase II	-
KB879_RS13065 (KB879_13065)	2752557..2753087	+	531	WP_211981635.1	riboflavin biosynthesis protein RibA	-
KB879_RS13070 (KB879_13070)	2753088..2753558	-	471	WP_010814564.1	Lrp/AsnC family transcriptional regulator	-
KB879_RS13075 (KB879_13075)	2753711..2754052	+	342	WP_041228660.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (25-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-68)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10605.23 Da
• Isoelectric Point: 9.9483

>T200022 WP_153953472.1 NZ_CP073677:2748944-2749222 [Cupriavidus sp. KK10]
MIQSFRCSDTAELFAGRCVPRFGNIRAVAERKLRLLQSASTLEFLRSPPGNRLQLLAGDRKGRYSIRINEQWRICFIWTH
DGPAAVEIIDYH

Nucleotide

• Length: 279 bp

>T200022 NZ_CP097688:914303-914710 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12307.13 Da
• Isoelectric Point: 8.6214

>AT200022 WP_035815276.1 NZ_CP073677:2749234-2749569 [Cupriavidus sp. KK10]
MVRFKDGMRPIHPGEILREDFLVPLNMSANALALALRVTAARINDIVREQRGITPDTALRLARYFGGDAQSWLNLQLDYD
LKVAQRDHGERIMAEVAPRNAAAAGQPAAEK

Nucleotide

• Length: 336 bp

>AT200022 NZ_CP097688:914056-914322 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA