Detailed information of TA system TA200022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2748944..2749569
• Replicon: chromosome
• Accession: NZ_CP073677
• Organism: Cupriavidus sp. KK10

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: KB879_RS13040
• Protein ID: WP_153953472.1
• Coordinates: 2748944..2749222 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KB879_RS13045
• Protein ID: WP_035815276.1
• Coordinates: 2749234..2749569 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (25-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-68)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10605.23 Da
• Isoelectric Point: 9.9483

>T200022 WP_153953472.1 NZ_CP073677:2748944-2749222 [Cupriavidus sp. KK10]
MIQSFRCSDTAELFAGRCVPRFGNIRAVAERKLRLLQSASTLEFLRSPPGNRLQLLAGDRKGRYSIRINEQWRICFIWTH
DGPAAVEIIDYH

Nucleotide

• Length: 279 bp

>T200022 NZ_CP097688:914303-914710 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12307.13 Da
• Isoelectric Point: 8.6214

>AT200022 WP_035815276.1 NZ_CP073677:2749234-2749569 [Cupriavidus sp. KK10]
MVRFKDGMRPIHPGEILREDFLVPLNMSANALALALRVTAARINDIVREQRGITPDTALRLARYFGGDAQSWLNLQLDYD
LKVAQRDHGERIMAEVAPRNAAAAGQPAAEK

Nucleotide

• Length: 336 bp

>AT200022 NZ_CP097688:914056-914322 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA