Detailed information of TA system TA199980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1631119..1631828
• Replicon: chromosome
• Accession: NZ_CP073655
• Organism: Nocardia seriolae strain TL20

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: KEC46_RS07385
• Protein ID: WP_033085761.1
• Coordinates: 1631119..1631502 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0B8N7P4
• Locus tag: KEC46_RS07390
• Protein ID: WP_071811585.1
• Coordinates: 1631499..1631828 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEC46_RS07360 (KEC46_07360)	1626186..1626695	+	510	WP_033085763.1	PaaI family thioesterase	-
KEC46_RS07365 (KEC46_07365)	1626897..1627202	-	306	WP_033085726.1	chorismate mutase	-
KEC46_RS07370 (KEC46_07370)	1627556..1629994	+	2439	WP_228102969.1	DNA helicase PcrA	-
KEC46_RS07375 (KEC46_07375)	1629995..1630234	-	240	WP_033085724.1	hypothetical protein	-
KEC46_RS07380 (KEC46_07380)	1630231..1630977	-	747	Protein_1460	cytochrome b	-
KEC46_RS07385 (KEC46_07385)	1631119..1631502	+	384	WP_033085761.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KEC46_RS07390 (KEC46_07390)	1631499..1631828	+	330	WP_071811585.1	helix-turn-helix transcriptional regulator	Antitoxin
KEC46_RS07395 (KEC46_07395)	1631898..1633280	-	1383	WP_071811584.1	DUF1731 domain-containing protein	-
KEC46_RS07400 (KEC46_07400)	1633470..1635092	-	1623	WP_033085722.1	acyl-CoA dehydrogenase family protein	-
KEC46_RS07405 (KEC46_07405)	1635106..1635735	-	630	WP_071343557.1	TetR/AcrR family transcriptional regulator	-
KEC46_RS07410 (KEC46_07410)	1635851..1636615	+	765	WP_033085720.1	crotonase/enoyl-CoA hydratase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-111)

Antitoxin

• HigA2-like, helix-turn-helix domain (41-98)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14590.47 Da
• Isoelectric Point: 4.4703

>T199980 WP_033085761.1 NZ_CP073655:1631119-1631502 [Nocardia seriolae]
VPGEISLLEPVDDWFMKLCESDPETAALVEQALDRLAEVGPTLGRPLVDTLEHSRLRNLKELRPGSRGSSEIRMLFVFDP
DREAIVLVAGDKAGRWTRWYEEAIPLAEHRYADYRAEKDMRQQEEQP

Nucleotide

• Length: 384 bp

>T199980 NZ_CP073655:1631119-1631502 [Nocardia seriolae]
GTGCCCGGGGAAATCTCGCTGCTCGAGCCGGTCGACGACTGGTTCATGAAGCTGTGCGAGTCGGATCCAGAAACGGCGGC
ATTGGTCGAGCAGGCGTTGGACAGGCTTGCCGAGGTGGGCCCCACGCTCGGTCGTCCACTCGTCGATACGCTGGAGCACA
GCCGACTACGCAATCTGAAGGAGCTGCGGCCCGGCTCCCGCGGTTCTTCGGAGATCCGGATGTTGTTTGTCTTCGACCCG
GACCGCGAAGCCATCGTTTTGGTCGCCGGCGACAAGGCCGGTCGGTGGACTCGTTGGTACGAAGAGGCGATTCCGTTGGC
TGAACATCGGTACGCGGACTATCGAGCCGAAAAAGACATGAGGCAGCAGGAGGAGCAGCCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11751.33 Da
• Isoelectric Point: 7.4681

>AT199980 WP_071811585.1 NZ_CP073655:1631499-1631828 [Nocardia seriolae]
MSPARNWRDIKAEAHRLHPELADPAVRAEAAAHLDAYIAGYHLKELRKAAGKTQADVAAALRVSQSRVSQIENGDLGAME
LDTLRAYAEALGGRVEITLSVGQHTIKVA

Nucleotide

• Length: 330 bp

>AT199980 NZ_CP073655:1631499-1631828 [Nocardia seriolae]
ATGAGCCCCGCGCGTAACTGGCGTGACATCAAGGCGGAAGCCCATCGCCTACATCCGGAACTGGCGGATCCCGCGGTACG
AGCCGAGGCGGCCGCACACCTCGATGCCTACATCGCGGGGTACCATCTGAAGGAACTCCGTAAGGCGGCGGGCAAGACCC
AAGCCGATGTTGCCGCCGCCCTTCGGGTTTCGCAGTCTCGTGTCTCGCAGATCGAGAACGGCGATCTCGGCGCAATGGAA
CTCGACACGCTCCGCGCCTACGCCGAAGCCCTTGGCGGTCGTGTCGAGATCACTCTCAGCGTCGGTCAGCATACGATCAA
GGTCGCCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0B8N7P4