Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2360450..2361139 Replicon chromosome
Accession NZ_CP073627
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2012_85054

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag KD921_RS11495 Protein ID WP_021469727.1
Coordinates 2360450..2360767 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag KD921_RS11500 Protein ID WP_020804705.1
Coordinates 2360843..2361139 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD921_RS11465 (2356152) 2356152..2356661 + 510 WP_020802144.1 GNAT family N-acetyltransferase -
KD921_RS11470 (2356671) 2356671..2357597 + 927 WP_032435210.1 amino acid ABC transporter permease -
KD921_RS11475 (2357581) 2357581..2358360 + 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
KD921_RS11480 (2358399) 2358399..2359250 + 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
KD921_RS11485 (2359328) 2359328..2359945 + 618 WP_032425015.1 glutathione S-transferase family protein -
KD921_RS11490 (2360016) 2360016..2360243 + 228 WP_002906690.1 tautomerase PptA -
KD921_RS11495 (2360450) 2360450..2360767 + 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KD921_RS11500 (2360843) 2360843..2361139 + 297 WP_020804705.1 NadS family protein Antitoxin
KD921_RS11505 (2361218) 2361218..2361664 + 447 WP_032435212.1 hypothetical protein -
KD921_RS11510 (2361705) 2361705..2363321 - 1617 WP_004175961.1 carbohydrate porin -
KD921_RS11515 (2363365) 2363365..2364747 - 1383 WP_004151222.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T199945 WP_021469727.1 NZ_CP073627:2360450-2360767 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

>T199945 NZ_CP097663:c5277004-5276621 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT199945 WP_020804705.1 NZ_CP073627:2360843-2361139 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp

>AT199945 NZ_CP097663:c5277246-5277004 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References