Detailed information of TA system TA199933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 37706..38433
• Replicon: plasmid unnamed1
• Accession: NZ_CP073626
• Organism: Escherichia coli strain AW1.7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A663AUB1
• Locus tag: KD735_RS24940
• Protein ID: WP_000558568.1
• Coordinates: 37706..38017 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: KD735_RS24945
• Protein ID: WP_000990392.1
• Coordinates: 38014..38433 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KD735_RS24920 (KD735_24665)	33105..33368	-	264	WP_001295708.1	hypothetical protein	-
KD735_RS24925 (KD735_24670)	33528..33764	+	237	Protein_36	transposase	-
KD735_RS24930 (KD735_24675)	33816..34520	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KD735_RS24935 (KD735_24680)	34585..37476	+	2892	Protein_38	Tn3 family transposase	-
KD735_RS24940 (KD735_24685)	37706..38017	+	312	WP_000558568.1	type II toxin-antitoxin system HigB family toxin	Toxin
KD735_RS24945 (KD735_24690)	38014..38433	+	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
KD735_RS24950	38470..39255	-	786	WP_001325310.1	hypothetical protein	-
KD735_RS25210	39263..39385	+	123	WP_001325309.1	hypothetical protein	-
KD735_RS24955 (KD735_24700)	39419..40070	-	652	Protein_43	IS6 family transposase	-
KD735_RS24960 (KD735_24705)	40061..40390	+	330	WP_109850802.1	hypothetical protein	-
KD735_RS24965 (KD735_24710)	40408..41217	+	810	WP_054623452.1	hypothetical protein	-
KD735_RS24970 (KD735_24715)	41506..42203	+	698	WP_225858595.1	IS1-like element IS1B family transposase	-
KD735_RS24975 (KD735_24720)	42403..42709	+	307	Protein_47	type II toxin-antitoxin system ParD family antitoxin	-
KD735_RS24980	42696..43026	+	331	Protein_48	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..75842	75842
	-	inside	IScluster/Tn	-	-	33816..52181	18365

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12372.11 Da
• Isoelectric Point: 10.0935

>T199933 WP_000558568.1 NZ_CP073626:37706-38017 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T199933 NZ_CP097662:c5383-4967 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT199933 WP_000990392.1 NZ_CP073626:38014-38433 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT199933 NZ_CP097662:c5610-5380 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A663AUB1

Antitoxin

• AlphaFold DB: V0AHC4