Detailed information of TA system TA199911

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1121920..1122647
• Replicon: chromosome
• Accession: NZ_CP073624
• Organism: Escherichia coli strain AW1.7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: KD735_RS05400
• Protein ID: WP_000547564.1
• Coordinates: 1121920..1122231 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KD735_RS05405
• Protein ID: WP_000126294.1
• Coordinates: 1122228..1122647 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KD735_RS05370 (1117062)	1117062..1118771	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
KD735_RS05375 (1118781)	1118781..1119323	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
KD735_RS05380 (1119323)	1119323..1120090	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
KD735_RS05385 (1120087)	1120087..1120497	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
KD735_RS05390 (1120490)	1120490..1120960	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
KD735_RS05395 (1120985)	1120985..1121746	+	762	WP_001026446.1	hypothetical protein	-
KD735_RS05400 (1121920)	1121920..1122231	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
KD735_RS05405 (1122228)	1122228..1122647	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
KD735_RS05410 (1122761)	1122761..1124185	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
KD735_RS05415 (1124194)	1124194..1125651	-	1458	WP_001107882.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
KD735_RS05420 (1125911)	1125911..1126921	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
KD735_RS05425 (1127070)	1127070..1127597	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T199911 WP_000547564.1 NZ_CP073624:1121920-1122231 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T199911 NZ_CP097658:876687-877094 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT199911 WP_000126294.1 NZ_CP073624:1122228-1122647 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT199911 NZ_CP097658:876440-876706 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5