Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 733993..734720 Replicon chromosome
Accession NZ_CP073624
Organism Escherichia coli strain AW1.7

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KD735_RS03550 Protein ID WP_000550189.1
Coordinates 733993..734307 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KD735_RS03555 Protein ID WP_000560266.1
Coordinates 734304..734720 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD735_RS03530 (730159) 730159..731145 - 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -
KD735_RS03535 (731224) 731224..731907 - 684 WP_001183040.1 vancomycin high temperature exclusion protein -
KD735_RS03540 (731984) 731984..732487 - 504 WP_001295542.1 M48 family metallopeptidase -
KD735_RS03545 (732572) 732572..733708 + 1137 WP_000018697.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KD735_RS03550 (733993) 733993..734307 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KD735_RS03555 (734304) 734304..734720 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KD735_RS03560 (734765) 734765..736783 - 2019 WP_000121421.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KD735_RS03565 (737109) 737109..739460 - 2352 WP_000695498.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T199907 WP_000550189.1 NZ_CP073624:733993-734307 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T199907 NZ_CP097656:32807-33091 [Klebsiella pneumoniae]
ATGAAAGAAGAACTTAAAAAATTTGATGTTGTTGAATTTCTTGAAGATGAAGAAGATATTCAGGAATATCTTAATGCGGC
AATTGAAGAAGATGATACAAAATATCTTTTTAAAGCATTAGGTAACATAGCCAGAGCAAGAAACATAAGTCAGCTATCTA
AAGAAGTTGGTATGTCCAGGGAGGGAATTTACAAGGCGTTATCCGGTGAAGGAAATCCATCATTCCAGACTGTCTCTAAA
ATCACAAAAGCACTTGGTTTAAAACTTCACTTCACAGGGGCTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT199907 WP_000560266.1 NZ_CP073624:734304-734720 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT199907 NZ_CP097656:32509-32805 [Klebsiella pneumoniae]
ATGATAGAAATAAAACGGACGCCGGAAGTTGAAAAGTGGCTCAGGTCTTTGAAGGACAGAACGGCAAAGGCAAAAATATT
GATGCGACTTGAGCGCATGGAGGACGGAAATTTTGGAGACGTGGAGCCAATAGGTGACGGATTATCAGAATTAAGGATTC
ATCAGGGTAAAGGCTACCGTGTGTATTTTGGAAACAAAAACAATCAAATTATTCTCTTGCTTTGTGGTGGAGATAAAAGC
ACGCAGCAAGCAGATATCAGAAAAGCGAAACAATTAGCTAAACAGTGGGGGTTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References