Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 689373..690172 Replicon chromosome
Accession NZ_CP073624
Organism Escherichia coli strain AW1.7

Toxin (Protein)


Gene name yhaV Uniprot ID D7XZ20
Locus tag KD735_RS03315 Protein ID WP_000347264.1
Coordinates 689373..689837 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KD735_RS03320 Protein ID WP_001307405.1
Coordinates 689837..690172 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD735_RS03285 (684374) 684374..684808 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KD735_RS03290 (684826) 684826..685704 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
KD735_RS03295 (685694) 685694..686473 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KD735_RS03300 (686484) 686484..686957 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KD735_RS03305 (686980) 686980..688260 - 1281 WP_054625916.1 tagatose-bisphosphate aldolase subunit KbaZ -
KD735_RS03310 (688509) 688509..689318 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KD735_RS03315 (689373) 689373..689837 - 465 WP_000347264.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KD735_RS03320 (689837) 689837..690172 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KD735_RS03325 (690321) 690321..691892 - 1572 WP_001273753.1 galactarate dehydratase -
KD735_RS03330 (692267) 692267..693601 + 1335 WP_054625915.1 galactarate/glucarate/glycerate transporter GarP -
KD735_RS03335 (693617) 693617..694387 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17894.29 Da        Isoelectric Point: 9.4945

>T199906 WP_000347264.1 NZ_CP073624:c689837-689373 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFDAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T199906 NZ_CP097656:32509-32805 [Klebsiella pneumoniae]
ATGATAGAAATAAAACGGACGCCGGAAGTTGAAAAGTGGCTCAGGTCTTTGAAGGACAGAACGGCAAAGGCAAAAATATT
GATGCGACTTGAGCGCATGGAGGACGGAAATTTTGGAGACGTGGAGCCAATAGGTGACGGATTATCAGAATTAAGGATTC
ATCAGGGTAAAGGCTACCGTGTGTATTTTGGAAACAAAAACAATCAAATTATTCTCTTGCTTTGTGGTGGAGATAAAAGC
ACGCAGCAAGCAGATATCAGAAAAGCGAAACAATTAGCTAAACAGTGGGGGTTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT199906 WP_001307405.1 NZ_CP073624:c690172-689837 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT199906 NZ_CP097656:32807-33091 [Klebsiella pneumoniae]
ATGAAAGAAGAACTTAAAAAATTTGATGTTGTTGAATTTCTTGAAGATGAAGAAGATATTCAGGAATATCTTAATGCGGC
AATTGAAGAAGATGATACAAAATATCTTTTTAAAGCATTAGGTAACATAGCCAGAGCAAGAAACATAAGTCAGCTATCTA
AAGAAGTTGGTATGTCCAGGGAGGGAATTTACAAGGCGTTATCCGGTGAAGGAAATCCATCATTCCAGACTGTCTCTAAA
ATCACAAAAGCACTTGGTTTAAAACTTCACTTCACAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QML2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References