Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4196470..4196972 Replicon chromosome
Accession NZ_CP073621
Organism Escherichia coli strain CE2050

Toxin (Protein)


Gene name yoeB Uniprot ID A0A0H2Z0A6
Locus tag KEW59_RS19760 Protein ID WP_000767826.1
Coordinates 4196718..4196972 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag KEW59_RS19755 Protein ID WP_001259255.1
Coordinates 4196470..4196721 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEW59_RS19730 (4191648) 4191648..4192715 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
KEW59_RS19735 (4192715) 4192715..4193785 - 1071 WP_064770503.1 histidinol-phosphate transaminase -
KEW59_RS19740 (4193782) 4193782..4195086 - 1305 WP_001350692.1 histidinol dehydrogenase -
KEW59_RS19745 (4195092) 4195092..4195991 - 900 WP_064770504.1 ATP phosphoribosyltransferase -
KEW59_RS19750 (4196137) 4196137..4196187 - 51 WP_001364200.1 his operon leader peptide -
KEW59_RS19755 (4196470) 4196470..4196721 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
KEW59_RS19760 (4196718) 4196718..4196972 + 255 WP_000767826.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
KEW59_RS19765 (4197055) 4197055..4197879 + 825 WP_000754737.1 SDR family oxidoreductase -
KEW59_RS19770 (4197925) 4197925..4198854 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
KEW59_RS19775 (4199069) 4199069..4199131 + 63 WP_010723108.1 membrane protein YoeI -
KEW59_RS19780 (4199121) 4199121..4200479 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
KEW59_RS19785 (4200658) 4200658..4201716 + 1059 WP_064770505.1 thiosulfate utilization transporter TsuA/YeeE -
KEW59_RS19790 (4201730) 4201730..4201957 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10195.64 Da        Isoelectric Point: 8.0353

>T199891 WP_000767826.1 NZ_CP073621:4196718-4196972 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRIIEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T199891 NZ_CP097654:834526-834744 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT199891 WP_001259255.1 NZ_CP073621:4196470-4196721 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT199891 NZ_CP097654:834125-834499 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2Z0A6

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References