Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2948799..2949598 Replicon chromosome
Accession NZ_CP073621
Organism Escherichia coli strain CE2050

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag KEW59_RS13965 Protein ID WP_000347270.1
Coordinates 2948799..2949263 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag KEW59_RS13970 Protein ID WP_001551693.1
Coordinates 2949263..2949598 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEW59_RS13935 (2943800) 2943800..2944234 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KEW59_RS13940 (2944252) 2944252..2945130 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KEW59_RS13945 (2945120) 2945120..2945899 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KEW59_RS13950 (2945910) 2945910..2946383 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KEW59_RS13955 (2946406) 2946406..2947686 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
KEW59_RS13960 (2947935) 2947935..2948744 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KEW59_RS13965 (2948799) 2948799..2949263 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KEW59_RS13970 (2949263) 2949263..2949598 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KEW59_RS13975 (2949747) 2949747..2951318 - 1572 WP_001273738.1 galactarate dehydratase -
KEW59_RS13980 (2951693) 2951693..2953027 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KEW59_RS13985 (2953043) 2953043..2953813 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T199886 WP_000347270.1 NZ_CP073621:c2949263-2948799 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T199886 NZ_CP097651:c4851397-4851113 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT199886 WP_001551693.1 NZ_CP073621:c2949598-2949263 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT199886 NZ_CP097651:c4851629-4851387 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References