Detailed information of TA system TA199885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2111880..2112482
• Replicon: chromosome
• Accession: NZ_CP073621
• Organism: Escherichia coli strain CE2050

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KEW59_RS09965
• Protein ID: WP_000897305.1
• Coordinates: 2112171..2112482 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KEW59_RS09960
• Protein ID: WP_000356395.1
• Coordinates: 2111880..2112170 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEW59_RS09925 (2107504)	2107504..2108406	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KEW59_RS09930 (2108403)	2108403..2109038	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KEW59_RS09935 (2109035)	2109035..2109964	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
KEW59_RS09940 (2110146)	2110146..2110388	-	243	WP_001309881.1	CopG family transcriptional regulator	-
KEW59_RS09945 (2110607)	2110607..2110825	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KEW59_RS09950 (2111244)	2111244..2111522	-	279	WP_001315112.1	hypothetical protein	-
KEW59_RS09955 (2111574)	2111574..2111795	-	222	WP_001550354.1	hypothetical protein	-
KEW59_RS09960 (2111880)	2111880..2112170	-	291	WP_000356395.1	NadS family protein	Antitoxin
KEW59_RS09965 (2112171)	2112171..2112482	-	312	WP_000897305.1	hypothetical protein	Toxin
KEW59_RS09970 (2112711)	2112711..2113619	+	909	WP_001550353.1	alpha/beta hydrolase	-
KEW59_RS09975 (2113787)	2113787..2114701	-	915	WP_109553727.1	transposase	-
KEW59_RS09980 (2114714)	2114714..2115601	-	888	Protein_1947	hypothetical protein	-
KEW59_RS09985 (2116017)	2116017..2116958	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KEW59_RS09990 (2117003)	2117003..2117440	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T199885 WP_000897305.1 NZ_CP073621:c2112482-2112171 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T199885 NZ_CP097651:c4684285-4683752 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT199885 WP_000356395.1 NZ_CP073621:c2112170-2111880 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT199885 NZ_CP097651:c4684562-4684296 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7