Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1151302..1151996 Replicon chromosome
Accession NZ_CP073621
Organism Escherichia coli strain CE2050

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag KEW59_RS05400 Protein ID WP_001263491.1
Coordinates 1151302..1151700 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag KEW59_RS05405 Protein ID WP_000554755.1
Coordinates 1151703..1151996 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (1147131) 1147131..1147211 - 81 NuclAT_10 - -
- (1147131) 1147131..1147211 - 81 NuclAT_10 - -
- (1147131) 1147131..1147211 - 81 NuclAT_10 - -
- (1147131) 1147131..1147211 - 81 NuclAT_10 - -
KEW59_RS05370 (1146471) 1146471..1147715 - 1245 WP_000189541.1 esterase FrsA -
KEW59_RS05375 (1147807) 1147807..1148265 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
KEW59_RS05380 (1148526) 1148526..1149983 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
KEW59_RS05385 (1150040) 1150040..1150392 - 353 Protein_1054 peptide chain release factor H -
KEW59_RS05390 (1150388) 1150388..1150594 - 207 Protein_1055 RtcB family protein -
KEW59_RS05395 (1150840) 1150840..1151292 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
KEW59_RS05400 (1151302) 1151302..1151700 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KEW59_RS05405 (1151703) 1151703..1151996 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KEW59_RS05410 (1152048) 1152048..1153103 - 1056 WP_001550655.1 DNA polymerase IV -
KEW59_RS05415 (1153174) 1153174..1153959 - 786 WP_074157994.1 putative lateral flagellar export/assembly protein LafU -
KEW59_RS05420 (1153931) 1153931..1155643 + 1713 Protein_1061 flagellar biosynthesis protein FlhA -
KEW59_RS05425 (1155748) 1155748..1156026 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KEW59_RS05430 (1156019) 1156019..1156375 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE 1099335..1151996 52661


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T199880 WP_001263491.1 NZ_CP073621:c1151700-1151302 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T199880 NZ_CP073621:c1151700-1151302 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT199880 WP_000554755.1 NZ_CP073621:c1151996-1151703 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT199880 NZ_CP073621:c1151996-1151703 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7


Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References