Detailed information of TA system TA199872

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83342..83595
• Replicon: plasmid pMFDS1006657
• Accession: NZ_CP073590
• Organism: Escherichia coli strain MFDS1006657

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: KD154_RS24760
• Protein ID: WP_001336447.1
• Coordinates: 83446..83595 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	83342..83398 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KD154_RS24730 (79335)	79335..80081	+	747	WP_024213675.1	type-F conjugative transfer system pilin acetylase TraX	-
KD154_RS24735 (80164)	80164..80532	+	369	WP_000722534.1	NirD/YgiW/YdeI family stress tolerance protein	-
KD154_RS24740 (80568)	80568..81125	+	558	WP_024213676.1	fertility inhibition protein FinO	-
KD154_RS24745 (81275)	81275..81478	+	204	WP_038811942.1	hypothetical protein	-
KD154_RS24750 (82221)	82221..82682	+	462	WP_053265357.1	thermonuclease family protein	-
KD154_RS25380 (83033)	83033..83167	+	135	WP_001058542.1	hypothetical protein	-
- (83342)	83342..83398	-	57	NuclAT_0	-	Antitoxin
- (83342)	83342..83398	-	57	NuclAT_0	-	Antitoxin
- (83342)	83342..83398	-	57	NuclAT_0	-	Antitoxin
- (83342)	83342..83398	-	57	NuclAT_0	-	Antitoxin
KD154_RS24760 (83446)	83446..83595	+	150	WP_001336447.1	Hok/Gef family protein	Toxin
KD154_RS24765 (83879)	83879..84136	+	258	WP_000084404.1	replication regulatory protein RepA	-
KD154_RS24770 (84371)	84371..84445	+	75	WP_001365705.1	RepA leader peptide Tap	-
KD154_RS24775 (84438)	84438..85295	+	858	WP_000130991.1	incFII family plasmid replication initiator RepA	-
KD154_RS24785 (86271)	86271..86510	+	240	WP_000859017.1	winged helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / espP	1..122855	122855

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T199872 WP_001336447.1 NZ_CP073590:83446-83595 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T199872 NZ_CP097647:c111167-110685 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Nucleotide

• Length: 57 bp

>AT199872 NZ_CP073590:c83398-83342 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin