Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 3448751..3449353 Replicon chromosome
Accession NZ_CP073589
Organism Escherichia coli strain MFDS1006657

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag KD154_RS16725 Protein ID WP_000897305.1
Coordinates 3448751..3449062 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KD154_RS16730 Protein ID WP_000356397.1
Coordinates 3449063..3449353 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD154_RS16695 (3443781) 3443781..3444566 + 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -
KD154_RS16700 (3444665) 3444665..3445264 + 600 WP_001295269.1 glucose-1-phosphatase -
KD154_RS16705 (3445258) 3445258..3446130 + 873 WP_000920762.1 virulence factor BrkB family protein -
KD154_RS16710 (3446127) 3446127..3446564 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
KD154_RS16715 (3446609) 3446609..3447550 + 942 WP_001343389.1 fatty acid biosynthesis protein FabY -
KD154_RS16720 (3447614) 3447614..3448522 - 909 WP_001162704.1 alpha/beta hydrolase -
KD154_RS16725 (3448751) 3448751..3449062 + 312 WP_000897305.1 hypothetical protein Toxin
KD154_RS16730 (3449063) 3449063..3449353 + 291 WP_000356397.1 NadS family protein Antitoxin
KD154_RS16735 (3449939) 3449939..3450157 + 219 WP_001295676.1 CopG family transcriptional regulator -
KD154_RS16740 (3450376) 3450376..3450618 + 243 WP_001086388.1 protein YiiF -
KD154_RS16745 (3450847) 3450847..3451827 - 981 WP_000399648.1 IS110-like element IS621 family transposase -
KD154_RS16750 (3452227) 3452227..3453156 - 930 WP_000027701.1 formate dehydrogenase accessory protein FdhE -
KD154_RS16755 (3453153) 3453153..3453788 - 636 WP_000829012.1 formate dehydrogenase cytochrome b556 subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3450847..3451827 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T199865 WP_000897305.1 NZ_CP073589:3448751-3449062 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T199865 NZ_CP097646:1810118-1810220 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT199865 WP_000356397.1 NZ_CP073589:3449063-3449353 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT199865 NZ_CP097646:c1810224-1810077 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References