Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 207049..207776 Replicon plasmid p00002
Accession NZ_CP073379
Organism Klebsiella sp. P1927

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag KB892_RS29900 Protein ID WP_004118600.1
Coordinates 207465..207776 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag KB892_RS29895 Protein ID WP_000990392.1
Coordinates 207049..207468 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KB892_RS29865 (KB892_29865) 202736..203098 - 363 WP_020324593.1 arsenite efflux transporter metallochaperone ArsD -
KB892_RS29870 (KB892_29870) 203146..203499 - 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KB892_RS31315 203792..203932 + 141 WP_004118614.1 hypothetical protein -
KB892_RS31320 204100..204408 + 309 WP_020324596.1 Ref family recombination enhancement nuclease -
KB892_RS29880 (KB892_29880) 204832..205491 + 660 WP_000078540.1 hypothetical protein -
KB892_RS29885 (KB892_29885) 205488..205817 + 330 WP_004118613.1 hypothetical protein -
KB892_RS29890 (KB892_29890) 205810..207012 + 1203 WP_003031541.1 hypothetical protein -
KB892_RS29895 (KB892_29895) 207049..207468 - 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
KB892_RS29900 (KB892_29900) 207465..207776 - 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
KB892_RS29905 (KB892_29905) 207987..208367 + 381 Protein_229 gluconate permease -
KB892_RS29910 (KB892_29910) 208417..209112 - 696 WP_004118595.1 lactate utilization protein C -
KB892_RS29915 (KB892_29915) 209105..210532 - 1428 WP_000023895.1 LutB/LldF family L-lactate oxidation iron-sulfur protein -
KB892_RS29920 (KB892_29920) 210543..211262 - 720 WP_001102107.1 (Fe-S)-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / blaLAP-2 / sul1 / qacE / dfrA1 / tet(A) / blaCTX-M-14 - 1..213917 213917


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T199831 WP_004118600.1 NZ_CP073379:c207776-207465 [Klebsiella sp. P1927]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T199831 NZ_CP097614:1804220-1804546 [Pasteurella multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAGCG
ATTTGCGAAGTCCACCAGGTAATAAATTGGAAAAATTGGAGCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAATATCGTTTAATATTTAAATATAAAGAAAGTAATTTTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT199831 WP_000990392.1 NZ_CP073379:c207468-207049 [Klebsiella sp. P1927]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT199831 NZ_CP097614:1804682-1805008 [Pasteurella multocida]
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCGAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGTCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTCGTCTATTGATCAGCATAATCTGTTAGAAAAAAT
CGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References