Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 7021..7667 Replicon chromosome
Accession NZ_CP073377
Organism Klebsiella sp. P1927

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag KB892_RS00025 Protein ID WP_002920560.1
Coordinates 7021..7368 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag KB892_RS00030 Protein ID WP_002920557.1
Coordinates 7368..7667 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KB892_RS00015 (KB892_00015) 2947..4380 + 1434 WP_002920564.1 glycogen synthase GlgA -
KB892_RS00020 (KB892_00020) 4398..6845 + 2448 WP_002920561.1 glycogen phosphorylase -
KB892_RS00025 (KB892_00025) 7021..7368 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KB892_RS00030 (KB892_00030) 7368..7667 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
KB892_RS00035 (KB892_00035) 7730..9238 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
KB892_RS00040 (KB892_00040) 9443..9772 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
KB892_RS00045 (KB892_00045) 9823..10653 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
KB892_RS00050 (KB892_00050) 10703..11461 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T199809 WP_002920560.1 NZ_CP073377:7021-7368 [Klebsiella sp. P1927]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T199809 NZ_CP097588:c1750265-1749915 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT199809 WP_002920557.1 NZ_CP073377:7368-7667 [Klebsiella sp. P1927]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT199809 NZ_CP097588:c1750552-1750271 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References