Detailed information of TA system TA199806

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27387..27640
• Replicon: plasmid p00004
• Accession: NZ_CP073376
• Organism: Klebsiella sp. P1954

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KB890_RS27310
• Protein ID: WP_001312851.1
• Coordinates: 27491..27640 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	27387..27446 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB890_RS27275 (22676)	22676..23380	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KB890_RS27280 (23432)	23432..24094	+	663	Protein_34	conjugative transfer relaxase/helicase TraI	-
KB890_RS27285 (24114)	24114..24860	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
KB890_RS27290 (24915)	24915..25475	+	561	WP_000139328.1	fertility inhibition protein FinO	-
KB890_RS27295 (25607)	25607..25807	+	201	WP_015059022.1	hypothetical protein	-
KB890_RS27300 (26193)	26193..26792	+	600	WP_032083981.1	PIN domain-containing protein	-
KB890_RS27305 (26854)	26854..27186	+	333	WP_152916585.1	hypothetical protein	-
- (27387)	27387..27446	-	60	NuclAT_1	-	Antitoxin
- (27387)	27387..27446	-	60	NuclAT_1	-	Antitoxin
- (27387)	27387..27446	-	60	NuclAT_1	-	Antitoxin
- (27387)	27387..27446	-	60	NuclAT_1	-	Antitoxin
KB890_RS27310 (27491)	27491..27640	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
KB890_RS27315 (27924)	27924..28172	+	249	WP_000083837.1	replication regulatory protein RepA	-
KB890_RS27660 (28287)	28287..28465	+	179	Protein_42	protein CopA/IncA	-
KB890_RS27325 (28484)	28484..29341	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
KB890_RS27330 (30280)	30280..30933	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
KB890_RS27335 (31026)	31026..31283	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KB890_RS27340 (31216)	31216..31617	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KB890_RS27345 (31866)	31866..32281	+	416	Protein_47	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B / rmtB	-	1..55826	55826

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T199806 WP_001312851.1 NZ_CP073376:27491-27640 [Klebsiella sp. P1954]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T199806 NZ_CP097587:c125322-124972 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT199806 NZ_CP073376:c27446-27387 [Klebsiella sp. P1954]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin