Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1570543..1571140 Replicon chromosome
Accession NZ_CP073373
Organism Klebsiella sp. P1954

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KB890_RS07670 Protein ID WP_004142563.1
Coordinates 1570823..1571140 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KB890_RS07665 Protein ID WP_004142561.1
Coordinates 1570543..1570830 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KB890_RS07635 (1566623) 1566623..1566871 + 249 WP_020325243.1 DUF1158 domain-containing protein -
KB890_RS07640 (1566889) 1566889..1567230 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KB890_RS07645 (1567261) 1567261..1568376 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
KB890_RS07650 (1568556) 1568556..1569137 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
KB890_RS07655 (1569137) 1569137..1569505 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
KB890_RS07660 (1569625) 1569625..1570278 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
KB890_RS07665 (1570543) 1570543..1570830 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KB890_RS07670 (1570823) 1570823..1571140 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KB890_RS07675 (1571325) 1571325..1572368 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
KB890_RS07680 (1573038) 1573038..1573904 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KB890_RS07685 (1574013) 1574013..1575440 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T199785 WP_004142563.1 NZ_CP073373:c1571140-1570823 [Klebsiella sp. P1954]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T199785 NZ_CP097576:843463-843861 [Microcystis aeruginosa str. Chao 1910]
GTGAGAATTTTACTGGATACACAATGCTGGCTGTGGTGGTTTGTCCAACCAGACAAGTTAAGTGAGAATGTAATTGAACA
AATTGCCAACGAAAGCAATGAAGTGTGGTTTTCTGTTGCCAGTGTTTGGGAGATGGGAATTAAAGTTTCAATCGGTAAAT
TGTCACTGCCAGAGCAAATAGATGATTATGTCTCTACTCGAATGACCCAACTAGGAGCTAGATCGTTAGAGATCAATGCT
TCTCATGCTTTGCGGGCGGCTGCATTGCCTTTACATCATCGTGATCCTTTTGACCGAATGCTGATTGCACAAGCTCAGGT
AGAAGATATGACACTTGTGAGTGCGGATTCAACATTTAATCAATATGAAGTGTCTTTGCTTTGGGCAGCTAGTTCTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT199785 WP_004142561.1 NZ_CP073373:c1570830-1570543 [Klebsiella sp. P1954]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT199785 NZ_CP097576:843221-843466 [Microcystis aeruginosa str. Chao 1910]
ATGGAAACTGTAAACATTCATCAGGCTAAAACAAGCCTTTCTCGGCTATTGTCCCGTGTGGAACTCGGAGAGGAAATCGT
TATTTCAAACCGAGGTATTCCAATTGCTAAACTTGTTCCATTTCGGACATCTTTAGATCGACGGTCTAGTTTAGGACAAG
ATCGAGGGATGTTTACCGTACCAGATGATTTTAATGCTCCTTTACCAGAAGATATTTTGGGAGCATTTGAGGGTGGTGCG
GAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References