Detailed information of TA system TA199779

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 77423..77692
• Replicon: plasmid plas2
• Accession: NZ_CP073362
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KDN44_RS24825
• Protein ID: WP_001372321.1
• Coordinates: 77567..77692 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	77423..77488 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDN44_RS24785	73004..73708	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KDN44_RS25805	74250..74419	+	170	Protein_81	hypothetical protein	-
KDN44_RS25810	74602..74682	-	81	Protein_82	hypothetical protein	-
KDN44_RS24790	74752..74958	+	207	WP_000275856.1	hypothetical protein	-
KDN44_RS24795	74984..75523	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
KDN44_RS24800	75591..75824	+	234	WP_000005987.1	DUF905 family protein	-
KDN44_RS24805	75852..76049	+	198	Protein_86	hypothetical protein	-
KDN44_RS24810	76104..76538	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
KDN44_RS24815	76535..77297	+	763	Protein_88	plasmid SOS inhibition protein A	-
KDN44_RS24820	77266..77454	-	189	WP_001299721.1	hypothetical protein	-
-	77266..77490	+	225	NuclAT_0	-	-
-	77266..77490	+	225	NuclAT_0	-	-
-	77266..77490	+	225	NuclAT_0	-	-
-	77266..77490	+	225	NuclAT_0	-	-
-	77423..77488	-	66	-	-	Antitoxin
KDN44_RS25815	77476..77625	+	150	Protein_90	plasmid maintenance protein Mok	-
KDN44_RS24825	77567..77692	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KDN44_RS25820	77912..78142	+	231	WP_071586998.1	hypothetical protein	-
KDN44_RS25825	78140..78312	-	173	Protein_93	hypothetical protein	-
KDN44_RS25830	78382..78588	+	207	WP_000547968.1	hypothetical protein	-
KDN44_RS24830	78613..78900	+	288	WP_000107535.1	hypothetical protein	-
KDN44_RS24835	79018..79839	+	822	WP_001234426.1	DUF932 domain-containing protein	-
KDN44_RS24840	80136..80726	-	591	WP_001376243.1	transglycosylase SLT domain-containing protein	-
KDN44_RS24845	81059..81442	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KDN44_RS24850	81629..82318	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aadA5 / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..134098	134098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T199779 WP_001372321.1 NZ_CP073362:77567-77692 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T199779 NZ_CP097575:526599-527177 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGTGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT199779 NZ_CP073362:c77488-77423 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin