Detailed information of TA system TA199775

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64356..64610
• Replicon: plasmid plas2
• Accession: NZ_CP073362
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KDN44_RS24720
• Protein ID: WP_001312851.1
• Coordinates: 64356..64505 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	64549..64610 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDN44_RS24685 (59908)	59908..60309	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KDN44_RS24690 (60242)	60242..60499	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KDN44_RS24695 (60592)	60592..61245	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
KDN44_RS24700 (62184)	62184..63041	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
KDN44_RS24705 (63034)	63034..63516	-	483	WP_001273588.1	hypothetical protein	-
KDN44_RS25795 (63509)	63509..63556	-	48	WP_229471593.1	hypothetical protein	-
KDN44_RS25800 (63547)	63547..63798	+	252	WP_223195197.1	replication protein RepA	-
KDN44_RS24715 (63815)	63815..64072	-	258	WP_000083845.1	replication regulatory protein RepA	-
KDN44_RS24720 (64356)	64356..64505	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (64549)	64549..64610	+	62	NuclAT_1	-	Antitoxin
- (64549)	64549..64610	+	62	NuclAT_1	-	Antitoxin
- (64549)	64549..64610	+	62	NuclAT_1	-	Antitoxin
- (64549)	64549..64610	+	62	NuclAT_1	-	Antitoxin
KDN44_RS24725 (64866)	64866..64940	-	75	Protein_68	endonuclease	-
KDN44_RS24730 (65186)	65186..65398	-	213	WP_001299730.1	ANR family transcriptional regulator	-
KDN44_RS24735 (65534)	65534..66094	-	561	WP_000139315.1	fertility inhibition protein FinO	-
KDN44_RS24740 (66197)	66197..67057	-	861	WP_000704514.1	alpha/beta hydrolase	-
KDN44_RS24745 (67116)	67116..67862	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
KDN44_RS24750 (67882)	67882..68571	-	690	Protein_73	conjugative transfer relaxase/helicase TraI	-
KDN44_RS24755 (68635)	68635..69339	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aadA5 / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..134098	134098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T199775 WP_001312851.1 NZ_CP073362:c64505-64356 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T199775 NZ_CP097572:c1221334-1220972 [Enterobacter kobei]
ATGTGGAATGTCGAGACAACGGACAGATTTGATCAATGGTACTTCGAACAAACAACAGCATTAAAAGAAGATGTGCTAGC
GATGATGCATATTCTTGCTGAGTTTGGCCCAACGCTTGGGCGGCCATACGTAGATACCGTGAAGGCTTCAGCCTATGCGA
ACATGAAGGAATTACGGATTCAACATGCGGGAAATCCCATCAGAGCTTTTTTTGCTTTTGATCCTGACCGTAAGGCAATT
GTGCTCTGTGCTGGAAACAAAACCGGTTGCAATCAAAAACGGTTTTATAACGAAATGATTACGCTGGCTGACGTTGAGTA
CAGCAGACATCTGGCGGATAAGGAGGCCATATGGCAACGCTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT199775 NZ_CP073362:64549-64610 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin