Detailed information of TA system TA199773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4671854..4672456
• Replicon: chromosome
• Accession: NZ_CP073360
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KDN44_RS22270
• Protein ID: WP_000897305.1
• Coordinates: 4672145..4672456 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KDN44_RS22265
• Protein ID: WP_000356397.1
• Coordinates: 4671854..4672144 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDN44_RS22240 (4667780)	4667780..4668682	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KDN44_RS22245 (4668679)	4668679..4669314	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KDN44_RS22250 (4669311)	4669311..4670240	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KDN44_RS22255 (4670570)	4670570..4670812	-	243	WP_001086388.1	protein YiiF	-
KDN44_RS22260 (4671031)	4671031..4671249	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KDN44_RS22265 (4671854)	4671854..4672144	-	291	WP_000356397.1	NadS family protein	Antitoxin
KDN44_RS22270 (4672145)	4672145..4672456	-	312	WP_000897305.1	hypothetical protein	Toxin
KDN44_RS22275 (4672685)	4672685..4673593	+	909	WP_001162704.1	alpha/beta hydrolase	-
KDN44_RS22280 (4673657)	4673657..4674598	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KDN44_RS22285 (4674643)	4674643..4675080	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KDN44_RS22290 (4675077)	4675077..4675949	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KDN44_RS22295 (4675943)	4675943..4676542	-	600	WP_001295269.1	glucose-1-phosphatase	-
KDN44_RS22300 (4676641)	4676641..4677426	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T199773 WP_000897305.1 NZ_CP073360:c4672456-4672145 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T199773 NZ_CP097572:c359992-359615 [Enterobacter kobei]
ATGCAAACACAACCTGTTCCTCCGAAACGGGAGGTTTCGCCACGCCCGTCACCCGTGGCGATCTGGCAGCGCCTCCTGAG
TCACCTGCTGGACCGTCACTACGGTCTGACGCTCAACGACACGCCATTTGGCAAAGATGGTGTGATCCAAGAGCATATCG
ATGCCGGTATCTCACTGTGCGACGCGGTGAATTTCATCGTGGAGAAATACGACCTGGTACGCACTGATCGTCGCGGTTTT
AACGCGGAAACTCAGTCGCCGCTTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGCTGATGACCCGTCA
TAGCTACAGGGCCGTTACCGACATCACCACCGGCAAATACCGTGAGGTGCAACCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT199773 WP_000356397.1 NZ_CP073360:c4672144-4671854 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT199773 NZ_CP097572:c360401-360042 [Enterobacter kobei]
TTGTCGAATACAATACCGCCTGTAAACCACGATATCGCCGCCCCTTGGTGGGGGCTTAAACGCGATATCACCCCCTGTTT
TGGCGCACGTCTGGTGCAGGAGGGCAACCGGCTGCATTACCTGAACGACCGCGCCAGCATCACCGGCACCTTCAGCGACG
CGGATTTACGCCATCTCGACCAGGCATTCCCGCTACTGCTGAAGCAACTGGAGCTGATGCTGCTTTCCGGTGAGCTGAAT
CCCCGTCATCAGCACTGCGTCACGCTGTATGCAAAAGGGCTGATATGCGAGGCCGACAGCCTCGGCTCGCACGGGTACGT
CTATCTCGCAATTTACCCGACACCTGCAACAACAGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7