Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3789232..3789773 Replicon chromosome
Accession NZ_CP073360
Organism Escherichia coli strain EC11

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KDN44_RS18190 Protein ID WP_000615976.1
Coordinates 3789495..3789773 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KDN44_RS18185 Protein ID WP_000729704.1
Coordinates 3789232..3789492 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KDN44_RS18160 (3785015) 3785015..3785800 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
KDN44_RS18165 (3785772) 3785772..3787484 + 1713 Protein_3586 flagellar biosynthesis protein FlhA -
KDN44_RS18170 (3787589) 3787589..3787867 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KDN44_RS18175 (3787860) 3787860..3788216 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
KDN44_RS18180 (3788273) 3788273..3789046 - 774 WP_000543897.1 C40 family peptidase -
KDN44_RS18185 (3789232) 3789232..3789492 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KDN44_RS18190 (3789495) 3789495..3789773 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KDN44_RS18195 (3789929) 3789929..3790669 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KDN44_RS18200 (3790640) 3790640..3791407 - 768 WP_000333380.1 class II glutamine amidotransferase -
KDN44_RS18205 (3791613) 3791613..3792191 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T199769 WP_000615976.1 NZ_CP073360:3789495-3789773 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T199769 NZ_CP097571:c2485176-2485069 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT199769 WP_000729704.1 NZ_CP073360:3789232-3789492 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT199769 NZ_CP097571:2484992-2485052 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References