Detailed information of TA system TA199714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1652985..1653679
• Replicon: chromosome
• Accession: NZ_CP073343
• Organism: Marinomonas rhizomae strain MB12-11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KDW99_RS07660
• Protein ID: WP_212915685.1
• Coordinates: 1653311..1653679 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KDW99_RS07655
• Protein ID: WP_212915686.1
• Coordinates: 1652985..1653299 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDW99_RS07630 (KDW99_07610)	1648273..1649151	+	879	WP_255828704.1	alpha/beta hydrolase	-
KDW99_RS07635 (KDW99_07615)	1649148..1649894	-	747	WP_255828705.1	WYL domain-containing protein	-
KDW99_RS07640 (KDW99_07620)	1649974..1650426	+	453	WP_255828706.1	GyrI-like domain-containing protein	-
KDW99_RS07645 (KDW99_07625)	1650566..1650994	+	429	WP_255828707.1	BLUF domain-containing protein	-
KDW99_RS07650 (KDW99_07630)	1651154..1652908	+	1755	WP_255828708.1	D-lactate dehydrogenase	-
KDW99_RS07655 (KDW99_07635)	1652985..1653299	-	315	WP_212915686.1	XRE family transcriptional regulator	Antitoxin
KDW99_RS07660 (KDW99_07640)	1653311..1653679	-	369	WP_212915685.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KDW99_RS07665 (KDW99_07645)	1653889..1655385	+	1497	WP_255828709.1	carboxypeptidase M32	-
KDW99_RS07670 (KDW99_07650)	1655397..1656314	-	918	WP_255828710.1	AraC family transcriptional regulator	-
KDW99_RS07675 (KDW99_07655)	1656419..1657153	+	735	WP_205114962.1	SDR family oxidoreductase	-
KDW99_RS07680 (KDW99_07660)	1657286..1657555	+	270	Protein_1507	carboxypeptidase M32	-
KDW99_RS07685 (KDW99_07665)	1657863..1658660	-	798	WP_255828711.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-84)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14308.40 Da
• Isoelectric Point: 7.9587

>T199714 WP_212915685.1 NZ_CP073343:c1653679-1653311 [Marinomonas rhizomae]
MIWEILITDEYEQWFLGLTDKEQEDVLSMVDVLEIKGPQLARPYADSIKGTNKVKNLKELRVQHAGKPYRVFYAFDPTRK
AILLCGGRKDGKKDKTFYDRYIPLAEKEFLNYLKNSDSENTR

Nucleotide

• Length: 369 bp

>T199714 NZ_CP097529:c1449533-1449066 [Pseudomonas putida]
ATGAGTGATGCGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTAATTGCCCACCCGCTGTTCCTGGCCAAACTGGA
CGCGCTAAGCGCTCAAGTCGAGGCTCAACGGGACAAAGACCCAACGGGTTATACGAAAAGAAATACCTTTAAAAGGCTGG
CCGCCATCCGGCGGTTGGCGTTCGATGTAATCCCTCAAGACCCGACCAAACCCGAATACCGGCAAGGGGCAACACTTGGC
GGTGACCACAAGCACTGGTTCAGAGCAAAGTTCTTCCAGCAGTACCGGTTGTTCTTCCGCTACCACACCCCCAGCAGGAT
CATCGTGCTGGCCTGGATCAATGACGAGTCGAGCAAGCGGGCCTATGAAAGTAGCGACGATGCTTACAAGGTCTTTCAAA
AGATGTTGCATAGCGGCAACCCACCGGACGATTGGGACCAGTTGCTGCAAGAGGCTGCCGCAGATTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11441.34 Da
• Isoelectric Point: 10.0512

>AT199714 WP_212915686.1 NZ_CP073343:c1653299-1652985 [Marinomonas rhizomae]
MAKSINELRKKIKPEVQAAARAKAVAIITEMSLAEVRKKRGINQADLAGLMNIAQPNISQIENRPDALISTLNQYIEALG
GKLEIHAKFPDGQDIEISQFSMVK

Nucleotide

• Length: 315 bp

>AT199714 NZ_CP097529:c1449856-1449530 [Pseudomonas putida]
ATGGCTGCCACCTTCGACGTCGAGTCCACGCTGACCGATCGCTATCAAACCACTGTTCCCGAAACGGTGCGGCGCGCCTT
GGGCTTGAAAAAACGTGACAAGATCCATTACTCGATTCGCCCCGACGGTGGGGTGATACTCACCCGCGCAGAGGCAACCG
AGGACGATCCGGTACTGGGCAAATTTCTGGATTTCCTGGCACACGATATCGCTACCAACCCTCAACGGCTGCAGGTTGTC
GATGCTGGCCTCATCGCGCGCCTCGACAAGCTTGTCGGCGACGCGGAGGTCGACCTGGATCAACCTCTGTCGGCGGACGA
TGAATGA