Detailed information of TA system TA199711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-DinJ
• Location: 173614..174099
• Replicon: chromosome
• Accession: NZ_CP073317
• Organism: Lacticaseibacillus rhamnosus strain CE1

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: KAW33_RS13765
• Protein ID: WP_249146496.1
• Coordinates: 173614..173721 (-)
• Length: 36 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A807RQZ2
• Locus tag: KAW33_RS00780
• Protein ID: WP_005684270.1
• Coordinates: 173827..174099 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAW33_RS00760 (KAW33_00760)	168931..169767	-	837	WP_014569137.1	Rgg/GadR/MutR family transcriptional regulator	-
KAW33_RS00765 (KAW33_00765)	169815..171230	-	1416	WP_015764852.1	IS5-like element ISLrh2 family transposase	-
KAW33_RS00770 (KAW33_00770)	171981..173390	-	1410	WP_211751532.1	3'-5' exonuclease	-
KAW33_RS13765	173614..173721	-	108	WP_249146496.1	hypothetical protein	Toxin
KAW33_RS00780 (KAW33_00780)	173827..174099	-	273	WP_005684270.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KAW33_RS00785 (KAW33_00785)	174212..174505	+	294	WP_005684269.1	metalloregulator ArsR/SmtB family transcription factor	-
KAW33_RS00790 (KAW33_00790)	174587..175750	+	1164	WP_005691640.1	MFS transporter	-
KAW33_RS00795 (KAW33_00795)	175904..176479	+	576	WP_211751533.1	DNA-3-methyladenine glycosylase I	-
KAW33_RS00800 (KAW33_00800)	176601..176819	+	219	WP_005691646.1	hypothetical protein	-
KAW33_RS00805 (KAW33_00805)	176948..177829	-	882	WP_005684263.1	class II fructose-1,6-bisphosphate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (6-97)

Antitoxin

• Antitoxin MqsA/HigA-2 (1-131)

Sequences

Toxin

Protein

• Length: 36 a.a.
• Molecular weight: 3982.71 Da
• Isoelectric Point: 4.6286

>T199711 WP_249146496.1 NZ_CP073317:c173721-173614 [Lacticaseibacillus rhamnosus]
MRVAPDWLLVYKVDAEALILMLLATGTHRDTLNIE

Nucleotide

• Length: 108 bp

>T199711 NZ_CP097528:c4750767-4750471 [Pseudomonas putida]
ATGGAAAAGAGAATGCCTCATTGCCCGCTGGAGCGGGTAAAAGCCCTGGCTGCGGCAAGGCGAATCCGCCCTACGGGTGC
TGCATTGAAAGGCGCCAAGGCGCTGGGAATGGATTTTCCGGGCATGCTTGAGGTGATCACCAGCTTGAAGCGCACTGATT
TCTACAAGAGCATGACCAGCCATATTGACCACCGGGTTTGGCAGGATGTGTATCGCCCGCTTACAGCAATTGGGTACGTT
TATTTGAAATTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10323.01 Da
• Isoelectric Point: 10.7322

>AT199711 WP_005684270.1 NZ_CP073317:c174099-173827 [Lacticaseibacillus rhamnosus]
MAKESRIRNWINTITRKRALHVLNRLGLERFSAINMYWKRIGDTGALPFTLEMSFADQLRFAEADVKAGRIKSFKTVGAL
MKDLYSDVDG

Nucleotide

• Length: 273 bp

>AT199711 NZ_CP097528:c4750468-4750067 [Pseudomonas putida]
ATGAGATGTCCAATATGCGGCGGCTCGGAGCTTGCACCTGACATTCAGGGCATGCCCTATAGCTACAAAGGCGAGATGAC
AGTGATTCCTGAGGTAAGTGGCGATTACTGCTCCGCATGTGGTGAATGTGTACTGAGTCATGATGAAGCCATGCGCGTCA
GTCACTTGATGACGGCATTCGAGCGCCAGGTCAACGCAAATGTTGTGGATCCTTCCTTTATTGCCTCCATACGCAGAAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATCTTCGGTGGTGGGGTCAATGCGTTCTCCCGTTATGAAAACGGCAAGAC
CACCCCGCCAGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAACTCTTTGAGGAAGTGCGTACTGCCT
GA

Structures

Antitoxin

• AlphaFold DB: A0A807RQZ2