Detailed information of TA system TA199684

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20507..20933
• Replicon: plasmid pSW1780-KPC
• Accession: NZ_CP073303
• Organism: Klebsiella pneumoniae strain SW1780

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KCX81_RS27220
• Protein ID: WP_001372321.1
• Coordinates: 20507..20632 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	20709..20933 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCX81_RS27185 (15562)	15562..15789	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
KCX81_RS27190 (15883)	15883..16569	-	687	WP_015059009.1	PAS domain-containing protein	-
KCX81_RS27195 (16760)	16760..17143	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KCX81_RS27200 (17420)	17420..18067	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
KCX81_RS27205 (18364)	18364..19185	-	822	WP_001234445.1	DUF932 domain-containing protein	-
KCX81_RS27210 (19296)	19296..19592	-	297	WP_001272251.1	hypothetical protein	-
KCX81_RS27215 (19892)	19892..20188	+	297	Protein_28	hypothetical protein	-
KCX81_RS27220 (20507)	20507..20632	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KCX81_RS28185 (20574)	20574..20723	-	150	Protein_30	plasmid maintenance protein Mok	-
- (20709)	20709..20933	-	225	NuclAT_0	-	Antitoxin
- (20709)	20709..20933	-	225	NuclAT_0	-	Antitoxin
- (20709)	20709..20933	-	225	NuclAT_0	-	Antitoxin
- (20709)	20709..20933	-	225	NuclAT_0	-	Antitoxin
KCX81_RS27225 (20945)	20945..21664	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
KCX81_RS27230 (21661)	21661..22095	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KCX81_RS27235 (22164)	22164..24187	-	2024	Protein_33	ParB/RepB/Spo0J family partition protein	-
KCX81_RS27240 (24248)	24248..24481	-	234	WP_000006003.1	DUF905 family protein	-
KCX81_RS27245 (24539)	24539..25066	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
KCX81_RS28190 (25368)	25368..25829	+	462	Protein_36	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / rmtB / blaTEM-1B / blaKPC-2 / catA2	-	1..125619	125619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T199684 WP_001372321.1 NZ_CP073303:c20632-20507 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T199684 NZ_CP097512:2106304-2106492 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT199684 NZ_CP073303:c20933-20709 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin