Detailed information of TA system TA199636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 98348..99005
• Replicon: plasmid unnamed2
• Accession: NZ_CP073264
• Organism: Duffyella gerundensis strain AR

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J9874_RS20410
• Protein ID: WP_225702135.1
• Coordinates: 98634..99005 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J9874_RS20405
• Protein ID: WP_225702134.1
• Coordinates: 98348..98647 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9874_RS20370 (J9874_04073)	93613..94239	+	627	WP_225702130.1	DUF2726 domain-containing protein	-
J9874_RS20375	95263..95496	+	234	Protein_86	plasmid SOS inhibition protein A	-
J9874_RS20380 (J9874_04074)	95544..95690	+	147	Protein_87	theronine dehydrogenase	-
J9874_RS20385	95712..95885	+	174	Protein_88	DDE-type integrase/transposase/recombinase	-
J9874_RS20390	95958..96470	+	513	WP_225702131.1	hypothetical protein	-
J9874_RS20395 (J9874_04076)	97816..97998	+	183	WP_225702132.1	hypothetical protein	-
J9874_RS20400 (J9874_04077)	98027..98290	+	264	WP_225702133.1	hypothetical protein	-
J9874_RS20405 (J9874_04078)	98348..98647	-	300	WP_225702134.1	XRE family transcriptional regulator	Antitoxin
J9874_RS20410 (J9874_04079)	98634..99005	-	372	WP_225702135.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J9874_RS20415 (J9874_04080)	99211..99606	+	396	WP_225702136.1	hypothetical protein	-
J9874_RS20420 (J9874_04081)	99689..100816	+	1128	WP_225702137.1	hypothetical protein	-
J9874_RS20425 (J9874_04082)	101410..101898	-	489	WP_225702138.1	hypothetical protein	-
J9874_RS20435 (J9874_04083)	102634..102927	-	294	WP_225702140.1	hypothetical protein	-
J9874_RS20440 (J9874_04084)	103032..103229	-	198	WP_225702141.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..107871	107871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14219.20 Da
• Isoelectric Point: 5.0279

>T199636 WP_225702135.1 NZ_CP073264:c99005-98634 [Duffyella gerundensis]
MAWKVVTRTFFDVWYEEQTDDVQEEIAAHLLILEEIGPQLGRPQVDQVKASKYKNMKELRVQVGGHPFRAFFAFDPERKA
IVLCAGDKKGEDEKLFYKRMIKTADIEFTSHLESPEIKDDGNA

Nucleotide

• Length: 372 bp

>T199636 NZ_CP097426:c3694248-3693850 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10921.83 Da
• Isoelectric Point: 10.3373

>AT199636 WP_225702134.1 NZ_CP073264:c98647-98348 [Duffyella gerundensis]
MATLKEMMAKRSPESRARIEARAAEIRQEITLAKLREELAVSQTHLAEVLGVKQPSVAKMEKVDNDPKLSTLRRYIAGLG
GELALDVKLPDGKRIALSL

Nucleotide

• Length: 300 bp

>AT199636 NZ_CP097426:c3694544-3694251 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA