Detailed information of TA system TA199576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3855355..3856041
• Replicon: chromosome
• Accession: NZ_CP073105
• Organism: Stutzerimonas stutzeri strain F2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KCX70_RS17770
• Protein ID: WP_049340868.1
• Coordinates: 3855688..3856041 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: W9TFC1
• Locus tag: KCX70_RS17765
• Protein ID: WP_037038764.1
• Coordinates: 3855355..3855684 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCX70_RS17750 (KCX70_17750)	3850528..3851532	+	1005	WP_212618305.1	IS5-like element ISPa16 family transposase	-
KCX70_RS17760 (KCX70_17760)	3854227..3854844	+	618	WP_020306044.1	HAD family hydrolase	-
KCX70_RS17765 (KCX70_17765)	3855355..3855684	-	330	WP_037038764.1	XRE family transcriptional regulator	Antitoxin
KCX70_RS17770 (KCX70_17770)	3855688..3856041	-	354	WP_049340868.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KCX70_RS17775 (KCX70_17775)	3856089..3856877	-	789	WP_212618306.1	tyrosine-type recombinase/integrase	-
KCX70_RS17785 (KCX70_17785)	3857398..3858030	+	633	WP_015275847.1	TetR/AcrR family transcriptional regulator	-
KCX70_RS17790 (KCX70_17790)	3858076..3859056	+	981	WP_212618308.1	MDR family oxidoreductase	-
KCX70_RS17795 (KCX70_17795)	3859289..3859942	-	654	WP_015275845.1	oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB	-
KCX70_RS17800 (KCX70_17800)	3859971..3860687	-	717	WP_212618309.1	glycine betaine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13479.90 Da
• Isoelectric Point: 4.8025

>T199576 WP_049340868.1 NZ_CP073105:c3856041-3855688 [Stutzerimonas stutzeri]
MTWDVEYTDEFGGWWESLTAEEQESIAVSVRLLEDRGPSLGFPHSSAINGARHGHMRELRTQHNGRPIRTLYAFDPRRSA
ILLIGGDKTGDNRWYEINVPLADRLYDEHLEQLEKEG

Nucleotide

• Length: 354 bp

>T199576 NZ_CP097411:c4714233-4713892 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12109.07 Da
• Isoelectric Point: 8.6213

>AT199576 WP_037038764.1 NZ_CP073105:c3855684-3855355 [Stutzerimonas stutzeri]
MAKKFADLRAKMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLAELLHVQQPSIAKMEKRTDMYISTLRSHIEAMG
GQLEVIARFPDGSVKISNFAEIEAAAQQA

Nucleotide

• Length: 330 bp

>AT199576 NZ_CP097411:c4714595-4714254 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Structures

Antitoxin

• AlphaFold DB: W9TFC1