Detailed information of TA system TA199563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6080690..6081285
• Replicon: chromosome
• Accession: NZ_CP073080
• Organism: Pseudomonas aeruginosa strain NDTH9845

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: KCG38_RS28560
• Protein ID: WP_003113526.1
• Coordinates: 6081007..6081285 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KCG38_RS28555
• Protein ID: WP_003099268.1
• Coordinates: 6080690..6080995 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCG38_RS28520 (KCG38_28520)	6075841..6076689	+	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
KCG38_RS28530 (KCG38_28530)	6076856..6077797	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
KCG38_RS28535 (KCG38_28535)	6077914..6078528	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
KCG38_RS28540 (KCG38_28540)	6078570..6079154	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
KCG38_RS28545 (KCG38_28545)	6079195..6080295	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
KCG38_RS28555 (KCG38_28555)	6080690..6080995	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
KCG38_RS28560 (KCG38_28560)	6081007..6081285	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KCG38_RS36250	6081338..6081466	-	129	Protein_5671	integrase	-
KCG38_RS28565 (KCG38_28565)	6081614..6083842	+	2229	WP_003099265.1	TonB-dependent receptor	-
KCG38_RS28570 (KCG38_28570)	6083912..6084559	-	648	WP_003095021.1	carbonate dehydratase	-
KCG38_RS28575 (KCG38_28575)	6084621..6085859	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T199563 WP_003113526.1 NZ_CP073080:c6081285-6081007 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T199563 NZ_CP097386:c93932-93489 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT199563 WP_003099268.1 NZ_CP073080:c6080995-6080690 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT199563 NZ_CP097386:c94159-93929 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: V6ALY3