Detailed information of TA system TA199526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 24181..24787
• Replicon: plasmid pSYSM
• Accession: NZ_CP073018
• Organism: Synechocystis sp. PCC 6803

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: KBZ93_RS16660
• Protein ID: WP_011153557.1
• Coordinates: 24181..24462 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KBZ93_RS16665
• Protein ID: WP_011153558.1
• Coordinates: 24473..24787 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBZ93_RS16630 (KBZ93_16635)	20523..21479	-	957	WP_011153551.1	LAGLIDADG endonuclease	-
KBZ93_RS16635 (KBZ93_16640)	21479..21760	-	282	WP_011153552.1	hypothetical protein	-
KBZ93_RS16640 (KBZ93_16645)	21843..23219	+	1377	WP_011153553.1	HNH endonuclease domain-containing protein	-
KBZ93_RS16645 (KBZ93_16650)	23255..23572	+	318	WP_011153554.1	nucleotidyltransferase domain-containing protein	-
KBZ93_RS16650 (KBZ93_16655)	23562..23903	+	342	WP_011153555.1	DUF86 domain-containing protein	-
KBZ93_RS16655 (KBZ93_16660)	23890..24099	+	210	WP_011153556.1	aminotransferase class V-fold PLP-dependent enzyme	-
KBZ93_RS16660 (KBZ93_16665)	24181..24462	+	282	WP_011153557.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KBZ93_RS16665 (KBZ93_16670)	24473..24787	+	315	WP_011153558.1	HigA family addiction module antitoxin	Antitoxin
KBZ93_RS16670 (KBZ93_16675)	24798..25955	+	1158	WP_011153559.1	cysteine desulfurase family protein	-
KBZ93_RS16675 (KBZ93_16680)	25994..27175	+	1182	WP_011153560.1	AAA family ATPase	-
KBZ93_RS16680 (KBZ93_16685)	27175..27735	+	561	WP_011153561.1	DUF4276 family protein	-
KBZ93_RS16685 (KBZ93_16690)	27859..28053	-	195	WP_011153562.1	DUF433 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..118712	118712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-84)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11075.72 Da
• Isoelectric Point: 10.3094

>T199526 WP_011153557.1 NZ_CP073018:24181-24462 [Synechocystis sp. PCC 6803]
VIRNFKDKEAQKIFERQRSRKLPGDIQQVALRKLRMLNRAETLQDLKVPPANRLEALKGDRQGQYSIRINAQWRICFQWE
NGEALDVEIIDYH

Nucleotide

• Length: 282 bp

>T199526 NZ_CP097370:c4314910-4314737 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11963.85 Da
• Isoelectric Point: 4.8359

>AT199526 WP_011153558.1 NZ_CP073018:24473-24787 [Synechocystis sp. PCC 6803]
MNPDKLKPIHPGEVLLEEFLQPMNLSQNKLALAIRVPARRINEIVQGKRRITADTALRLALYFNMSPRFWLGLQMDYDLD
LAEDEIGEKLQQEVTTFNLDNVAV

Nucleotide

• Length: 315 bp

>AT199526 NZ_CP097370:c4315234-4314938 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG