Detailed information of TA system TA199481

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 132166..132419
• Replicon: plasmid unnamed1
• Accession: NZ_CP073003
• Organism: Klebsiella pneumoniae strain FO15

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KBT91_RS27730
• Protein ID: WP_001312851.1
• Coordinates: 132270..132419 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	132166..132225 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBT91_RS27690 (127826)	127826..127891	-	66	Protein_173	helix-turn-helix domain-containing protein	-
KBT91_RS27695 (127944)	127944..128648	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KBT91_RS27700 (128673)	128673..128873	+	201	WP_072354025.1	hypothetical protein	-
KBT91_RS27705 (128893)	128893..129639	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
KBT91_RS27710 (129694)	129694..130254	+	561	WP_000139328.1	fertility inhibition protein FinO	-
KBT91_RS27715 (130386)	130386..130586	+	201	WP_015059022.1	hypothetical protein	-
KBT91_RS27720 (130972)	130972..131571	+	600	WP_032083981.1	PIN domain-containing protein	-
KBT91_RS27725 (131633)	131633..131965	+	333	WP_152916585.1	hypothetical protein	-
- (132166)	132166..132225	-	60	NuclAT_1	-	Antitoxin
- (132166)	132166..132225	-	60	NuclAT_1	-	Antitoxin
- (132166)	132166..132225	-	60	NuclAT_1	-	Antitoxin
- (132166)	132166..132225	-	60	NuclAT_1	-	Antitoxin
KBT91_RS27730 (132270)	132270..132419	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
KBT91_RS27735 (132703)	132703..132951	+	249	WP_000083837.1	replication regulatory protein RepA	-
KBT91_RS28430 (133196)	133196..133270	+	75	WP_001375168.1	RepA leader peptide Tap	-
KBT91_RS27750 (133263)	133263..134120	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
KBT91_RS27755 (135059)	135059..135712	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
KBT91_RS27760 (135805)	135805..136062	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KBT91_RS27765 (135995)	135995..136396	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KBT91_RS27770 (136645)	136645..137060	+	416	Protein_188	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 / catA2 / blaCTX-M-65	-	1..144365	144365

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T199481 WP_001312851.1 NZ_CP073003:132270-132419 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T199481 NZ_CP097360:4524674-4525051 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACCCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATGACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT199481 NZ_CP073003:c132225-132166 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin