Detailed information of TA system TA199477

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25898..26167
• Replicon: plasmid unnamed1
• Accession: NZ_CP073003
• Organism: Klebsiella pneumoniae strain FO15

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KBT91_RS27000
• Protein ID: WP_001372321.1
• Coordinates: 26042..26167 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	25898..25963 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBT91_RS26970	21608..22135	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
KBT91_RS26975	22193..22426	+	234	WP_000006003.1	DUF905 family protein	-
KBT91_RS26980	22487..24510	+	2024	Protein_31	ParB/RepB/Spo0J family partition protein	-
KBT91_RS26985	24579..25013	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KBT91_RS26990	25010..25729	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	25741..25965	+	225	NuclAT_0	-	-
-	25741..25965	+	225	NuclAT_0	-	-
-	25741..25965	+	225	NuclAT_0	-	-
-	25741..25965	+	225	NuclAT_0	-	-
-	25898..25963	-	66	-	-	Antitoxin
KBT91_RS26995	25951..26100	+	150	Protein_34	plasmid maintenance protein Mok	-
KBT91_RS27000	26042..26167	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KBT91_RS27005	26486..26782	-	297	Protein_36	hypothetical protein	-
KBT91_RS27010	27082..27378	+	297	WP_001272251.1	hypothetical protein	-
KBT91_RS27015	27489..28310	+	822	WP_001234445.1	DUF932 domain-containing protein	-
KBT91_RS27020	28607..29254	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
KBT91_RS27025	29531..29914	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KBT91_RS27030	30105..30791	+	687	WP_015059009.1	PAS domain-containing protein	-
KBT91_RS27035	30885..31112	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 / catA2 / blaCTX-M-65	-	1..144365	144365

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T199477 WP_001372321.1 NZ_CP073003:26042-26167 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T199477 NZ_CP097360:c3803251-3802331 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT199477 NZ_CP073003:c25963-25898 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin