Detailed information of TA system TA199454

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 123518..123771
• Replicon: plasmid p1878-163k
• Accession: NZ_CP072998
• Organism: Klebsiella pneumoniae strain KP1878

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KA532_RS28710
• Protein ID: WP_001312851.1
• Coordinates: 123622..123771 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	123518..123577 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KA532_RS28670 (119178)	119178..119243	-	66	Protein_155	helix-turn-helix domain-containing protein	-
KA532_RS28675 (119296)	119296..120000	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KA532_RS28680 (120025)	120025..120225	+	201	WP_072354025.1	hypothetical protein	-
KA532_RS28685 (120245)	120245..120991	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
KA532_RS28690 (121046)	121046..121606	+	561	WP_000139328.1	fertility inhibition protein FinO	-
KA532_RS28695 (121738)	121738..121938	+	201	WP_015059022.1	hypothetical protein	-
KA532_RS28700 (122324)	122324..122923	+	600	WP_032083981.1	PIN domain-containing protein	-
KA532_RS28705 (122985)	122985..123317	+	333	WP_152916585.1	hypothetical protein	-
- (123518)	123518..123577	-	60	NuclAT_1	-	Antitoxin
- (123518)	123518..123577	-	60	NuclAT_1	-	Antitoxin
- (123518)	123518..123577	-	60	NuclAT_1	-	Antitoxin
- (123518)	123518..123577	-	60	NuclAT_1	-	Antitoxin
KA532_RS28710 (123622)	123622..123771	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
KA532_RS28715 (124055)	124055..124303	+	249	WP_000083837.1	replication regulatory protein RepA	-
KA532_RS29435 (124418)	124418..124596	+	179	Protein_165	protein CopA/IncA	-
KA532_RS28725 (124615)	124615..125472	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
KA532_RS28730 (126411)	126411..127064	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
KA532_RS28735 (127157)	127157..127414	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KA532_RS28740 (127347)	127347..127748	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KA532_RS28745 (127997)	127997..128412	+	416	Protein_170	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA2 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB	-	1..163348	163348

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T199454 WP_001312851.1 NZ_CP072998:123622-123771 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T199454 NZ_CP097351:241195-241545 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT199454 NZ_CP072998:c123577-123518 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin