Detailed information of TA system TA199420

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3961123..3961535
• Replicon: chromosome
• Accession: NZ_CP072986
• Organism: Escherichia coli strain T159A

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: KBW97_RS19290
• Protein ID: WP_000132601.1
• Coordinates: 3961194..3961535 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3961123..3961199 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW97_RS19280 (3957986)	3957986..3959575	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
KBW97_RS19285 (3959572)	3959572..3960966	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
- (3961123)	3961123..3961199	-	77	NuclAT_14	-	Antitoxin
- (3961123)	3961123..3961199	-	77	NuclAT_14	-	Antitoxin
- (3961123)	3961123..3961199	-	77	NuclAT_14	-	Antitoxin
- (3961123)	3961123..3961199	-	77	NuclAT_14	-	Antitoxin
- (3961123)	3961123..3961199	-	77	NuclAT_15	-	Antitoxin
- (3961123)	3961123..3961199	-	77	NuclAT_15	-	Antitoxin
- (3961123)	3961123..3961199	-	77	NuclAT_15	-	Antitoxin
- (3961123)	3961123..3961199	-	77	NuclAT_15	-	Antitoxin
KBW97_RS19290 (3961194)	3961194..3961535	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
KBW97_RS19295 (3961697)	3961697..3963076	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
KBW97_RS19300 (3963076)	3963076..3964122	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T199420 WP_000132601.1 NZ_CP072986:3961194-3961535 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T199420 NZ_CP097338:c1891665-1891369 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT199420 NZ_CP072986:c3961199-3961123 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin