Detailed information of TA system TA199418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3646683..3647377
• Replicon: chromosome
• Accession: NZ_CP072986
• Organism: Escherichia coli strain T159A

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: KBW97_RS17815
• Protein ID: WP_001263489.1
• Coordinates: 3646683..3647081 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: KBW97_RS17820
• Protein ID: WP_000554758.1
• Coordinates: 3647084..3647377 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3642271)	3642271..3642351	-	81	NuclAT_12	-	-
- (3642271)	3642271..3642351	-	81	NuclAT_12	-	-
- (3642271)	3642271..3642351	-	81	NuclAT_12	-	-
- (3642271)	3642271..3642351	-	81	NuclAT_12	-	-
KBW97_RS17790 (3642947)	3642947..3643405	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KBW97_RS17795 (3643666)	3643666..3645123	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
KBW97_RS17800 (3645180)	3645180..3645701	-	522	Protein_3526	peptide chain release factor H	-
KBW97_RS17805 (3645697)	3645697..3645903	-	207	Protein_3527	RtcB family protein	-
KBW97_RS17810 (3646221)	3646221..3646673	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
KBW97_RS17815 (3646683)	3646683..3647081	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KBW97_RS17820 (3647084)	3647084..3647377	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KBW97_RS17825 (3647429)	3647429..3648484	-	1056	WP_001226164.1	DNA polymerase IV	-
KBW97_RS17830 (3648555)	3648555..3649340	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
KBW97_RS17835 (3649312)	3649312..3651024	+	1713	Protein_3533	flagellar biosynthesis protein FlhA	-
KBW97_RS17840 (3651248)	3651248..3651745	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T199418 WP_001263489.1 NZ_CP072986:c3647081-3646683 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T199418 NZ_CP097338:c1100659-1100477 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT199418 WP_000554758.1 NZ_CP072986:c3647377-3647084 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT199418 NZ_CP097338:c1100438-1100019 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3