Detailed information of TA system TA199398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 720455..721148
• Replicon: chromosome
• Accession: NZ_CP072986
• Organism: Escherichia coli strain T159A

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: KBW97_RS03475
• Protein ID: WP_000415584.1
• Coordinates: 720455..720751 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: KBW97_RS03480
• Protein ID: WP_000650107.1
• Coordinates: 720753..721148 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW97_RS03440 (715543)	715543..715857	-	315	WP_000958598.1	putative quinol monooxygenase	-
KBW97_RS03445 (715888)	715888..716469	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
KBW97_RS03450 (716788)	716788..717120	+	333	WP_000917684.1	DUF2645 family protein	-
KBW97_RS03455 (717166)	717166..718515	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
KBW97_RS03460 (718512)	718512..719171	-	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	-
KBW97_RS03465 (719323)	719323..719715	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
KBW97_RS03470 (719768)	719768..720250	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
KBW97_RS03475 (720455)	720455..720751	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
KBW97_RS03480 (720753)	720753..721148	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
KBW97_RS03485 (721281)	721281..722888	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
KBW97_RS03490 (723026)	723026..725284	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T199398 WP_000415584.1 NZ_CP072986:720455-720751 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T199398 NZ_CP097336:c824495-824220 [Mannheimia haemolytica]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACATTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCTAATCCTGATACAGATAAGGGGGCAGTAGCGAGTATTAAAAAA
TGGTTATCTGAAAACGGAGTCATTCCTTATGCGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT199398 WP_000650107.1 NZ_CP072986:720753-721148 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT199398 NZ_CP097336:c824227-823898 [Mannheimia haemolytica]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTTATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGGGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65