Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 611199..611998 Replicon chromosome
Accession NZ_CP072986
Organism Escherichia coli strain T159A

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KBW97_RS02945 Protein ID WP_000347273.1
Coordinates 611199..611663 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KBW97_RS02950 Protein ID WP_001307405.1
Coordinates 611663..611998 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW97_RS02920 (607375) 607375..607932 - 558 Protein_580 amidohydrolase family protein -
KBW97_RS02925 (607928) 607928..608299 - 372 Protein_581 PTS sugar transporter subunit IIC -
KBW97_RS02930 (608310) 608310..608783 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KBW97_RS02935 (608806) 608806..610086 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KBW97_RS02940 (610335) 610335..611144 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KBW97_RS02945 (611199) 611199..611663 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KBW97_RS02950 (611663) 611663..611998 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KBW97_RS02955 (612147) 612147..613718 - 1572 WP_001273753.1 galactarate dehydratase -
KBW97_RS02960 (614093) 614093..615427 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KBW97_RS02965 (615443) 615443..616213 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 600215..622873 22658


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T199396 WP_000347273.1 NZ_CP072986:c611663-611199 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T199396 NZ_CP097336:295061-295330 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GCTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT199396 WP_001307405.1 NZ_CP072986:c611998-611663 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT199396 NZ_CP097336:294808-295074 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACCTTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References