Detailed information of TA system TA199388

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14473..14712
• Replicon: plasmid pEC2547-3
• Accession: NZ_CP072982
• Organism: Escherichia coli strain 2547

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: KBW66_RS26020
• Protein ID: WP_023144756.1
• Coordinates: 14473..14607 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	14652..14712 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW66_RS25980 (9820)	9820..10235	-	416	Protein_14	IS1-like element IS1B family transposase	-
KBW66_RS25985 (10484)	10484..10885	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KBW66_RS25990 (10818)	10818..11075	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KBW66_RS25995 (11168)	11168..11821	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
KBW66_RS26000 (12761)	12761..13618	-	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
KBW66_RS26005 (13611)	13611..13685	-	75	WP_031943482.1	RepA leader peptide Tap	-
KBW66_RS26015 (13922)	13922..14176	-	255	WP_000083850.1	replication regulatory protein RepA	-
KBW66_RS26020 (14473)	14473..14607	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (14652)	14652..14712	+	61	NuclAT_2	-	Antitoxin
- (14652)	14652..14712	+	61	NuclAT_2	-	Antitoxin
- (14652)	14652..14712	+	61	NuclAT_2	-	Antitoxin
- (14652)	14652..14712	+	61	NuclAT_2	-	Antitoxin
KBW66_RS26025 (14679)	14679..14965	-	287	Protein_22	DUF2726 domain-containing protein	-
KBW66_RS26030 (15043)	15043..16656	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
KBW66_RS26035 (16687)	16687..17037	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
KBW66_RS26040 (17034)	17034..17459	-	426	WP_000422741.1	transposase	-
KBW66_RS26045 (18018)	18018..18230	-	213	WP_013023861.1	hypothetical protein	-
KBW66_RS26050 (18361)	18361..18921	-	561	WP_000139341.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / tet(A) / blaCTX-M-27 / sul2	-	1..81323	81323
	-	inside	IScluster/Tn	aph(6)-Id / tet(A) / blaCTX-M-27	-	246..17459	17213

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T199388 WP_023144756.1 NZ_CP072982:c14607-14473 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T199388 NZ_CP097334:c1894283-1893987 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT199388 NZ_CP072982:14652-14712 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin