Detailed information of TA system TA199387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 79356..80007
• Replicon: plasmid pEC2547-KPC-2
• Accession: NZ_CP072981
• Organism: Escherichia coli strain 2547

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KBW66_RS25790
• Protein ID: WP_094283547.1
• Coordinates: 79356..79706 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KBW66_RS25795
• Protein ID: WP_094283546.1
• Coordinates: 79708..80007 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW66_RS25760 (KBW66_25760)	74824..75558	+	735	WP_094283552.1	MobC family replication-relaxation protein	-
KBW66_RS25765 (KBW66_25765)	75562..76041	+	480	WP_094283551.1	thermonuclease family protein	-
KBW66_RS25770 (KBW66_25770)	76271..76636	+	366	WP_000124098.1	hypothetical protein	-
KBW66_RS25775 (KBW66_25775)	76636..77823	+	1188	WP_000937614.1	IS91-like element IS91 family transposase	-
KBW66_RS25780 (KBW66_25780)	78073..78306	-	234	WP_000024515.1	hypothetical protein	-
KBW66_RS25785 (KBW66_25785)	78346..78993	-	648	WP_000214917.1	ParA family protein	-
KBW66_RS25790 (KBW66_25790)	79356..79706	+	351	WP_094283547.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KBW66_RS25795 (KBW66_25795)	79708..80007	+	300	WP_094283546.1	helix-turn-helix transcriptional regulator	Antitoxin
KBW66_RS25800 (KBW66_25800)	80014..80124	+	111	Protein_105	transcriptional regulator	-
KBW66_RS25805 (KBW66_25805)	80199..80438	-	240	WP_094283545.1	hypothetical protein	-
KBW66_RS25810 (KBW66_25810)	80428..80685	-	258	WP_024192529.1	hypothetical protein	-
KBW66_RS25815 (KBW66_25815)	80722..81264	-	543	WP_094283544.1	hypothetical protein	-
KBW66_RS25820 (KBW66_25820)	81392..81925	-	534	WP_001025389.1	J domain-containing protein	-
KBW66_RS25825 (KBW66_25825)	82296..82595	-	300	WP_001615573.1	hypothetical protein	-
KBW66_RS25830 (KBW66_25830)	82669..82941	-	273	WP_094283543.1	hypothetical protein	-
KBW66_RS25835 (KBW66_25835)	83900..84613	-	714	WP_223228604.1	RepA family replication protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / ARR-3 / aac(6')-Ib-cr / qnrS1 / dfrA14 / blaTEM-1B / blaCTX-M-3	-	1..94462	94462
	-	flank	IS/Tn	-	-	76636..77823	1187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (17-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13408.35 Da
• Isoelectric Point: 6.9806

>T199387 WP_094283547.1 NZ_CP072981:79356-79706 [Escherichia coli]
MWNVVTTDIFDAWFLTQNEDLRESVYEAMGLLEKFGPTLGRPYVDTLNGSDFANMKELRVQYKGSPVRAFFAFDPTRNAI
VLCAGDKTGLNEKRFYKDMIKLADSEYRKHLAKLEK

Nucleotide

• Length: 351 bp

>T199387 NZ_CP097334:1416041-1416217 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10986.70 Da
• Isoelectric Point: 8.5545

>AT199387 WP_094283546.1 NZ_CP072981:79708-80007 [Escherichia coli]
MGTYRELLAKESPEMQARVAERVEKASVQIALSALRDELNISQTELATMMGVKQPSVARMEQTDNDPRISTLKRYVKALG
GELSLDVTLPTGKRVAFHL

Nucleotide

• Length: 300 bp

>AT199387 NZ_CP097334:1416273-1416689 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA