Detailed information of TA system TA199385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 4156425..4156927
• Replicon: chromosome
• Accession: NZ_CP072980
• Organism: Escherichia coli strain 2547

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: KBW66_RS20610
• Protein ID: WP_000767819.1
• Coordinates: 4156425..4156679 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: KBW66_RS20615
• Protein ID: WP_001259253.1
• Coordinates: 4156676..4156927 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW66_RS20585 (4152243)	4152243..4152701	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
KBW66_RS20590 (4152918)	4152918..4154276	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
KBW66_RS20595 (4154266)	4154266..4154328	-	63	WP_010723108.1	membrane protein YoeI	-
KBW66_RS20600 (4154543)	4154543..4155472	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
KBW66_RS20605 (4155518)	4155518..4156342	-	825	WP_000754737.1	SDR family oxidoreductase	-
KBW66_RS20610 (4156425)	4156425..4156679	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KBW66_RS20615 (4156676)	4156676..4156927	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KBW66_RS20620 (4157210)	4157210..4157260	+	51	WP_001364200.1	his operon leader peptide	-
KBW66_RS20625 (4157406)	4157406..4158305	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KBW66_RS20630 (4158311)	4158311..4159615	+	1305	WP_001296211.1	histidinol dehydrogenase	-
KBW66_RS20635 (4159612)	4159612..4160682	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
KBW66_RS20640 (4160682)	4160682..4161749	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4152243..4152701	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T199385 WP_000767819.1 NZ_CP072980:c4156679-4156425 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T199385 NZ_CP097334:c883151-882882 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT199385 WP_001259253.1 NZ_CP072980:c4156927-4156676 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT199385 NZ_CP097334:c883421-883155 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3