Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 2402920..2403599 Replicon chromosome
Accession NZ_CP072980
Organism Escherichia coli strain 2547

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag KBW66_RS11540 Protein ID WP_000057523.1
Coordinates 2402920..2403222 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag KBW66_RS11545 Protein ID WP_000806442.1
Coordinates 2403258..2403599 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW66_RS11515 (2398296) 2398296..2399948 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
KBW66_RS11520 (2399986) 2399986..2400489 - 504 WP_000667000.1 hypothetical protein -
KBW66_RS11525 (2400486) 2400486..2401142 - 657 WP_015674862.1 hypothetical protein -
KBW66_RS11530 (2401310) 2401310..2401789 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
KBW66_RS11535 (2401993) 2401993..2402787 - 795 WP_000365147.1 TraB/GumN family protein -
KBW66_RS11540 (2402920) 2402920..2403222 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KBW66_RS11545 (2403258) 2403258..2403599 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
KBW66_RS11550 (2403657) 2403657..2406161 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
KBW66_RS11555 (2406423) 2406423..2407355 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T199370 WP_000057523.1 NZ_CP072980:2402920-2403222 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T199370 NZ_CP097330:899100-899378 [Cupriavidus campinensis]
ATGATTCGCTCATTTGCGCACAAGGGTCTCGAACGCTTCTTCACCACAGGCAGCACGGCGGCGATTCCCGCGATGCACGT
CAAGCGGTTGCGGCTGATCCTGGCGCTGCTGGACGAGGCAGGCTCCGCGCGCGATATGGATGCGCCCGGCTTGCGACTGC
ACGCGCTCAAGGGCGACCGCGCGGAGCTTTGGGCCGTGACGGTGCAGGCGAACTGGCGGGTGACCTTCCGGTTCGAGAAC
GGGGACGCGCACATCGTCAACTACATTGACTATCACTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT199370 WP_000806442.1 NZ_CP072980:2403258-2403599 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT199370 NZ_CP097330:899392-899682 [Cupriavidus campinensis]
ATGCGCATGCACAATCCACCGCATCCGGGCGAGTTGCTTGCGGAGCTTTGGCTCGAGCCGCTGCGGCTGACGATTACCGA
TACCGCCGCGGCGCTCGATGTTTCGCGGAAGACGGTTTCCGAGATCGTGAATGGCCGGGCTGGCGTCACGCCGGAGATGG
CGGTTCGGCTTGAACTGGCGTTCGGCAAGAGCGCCGAGTCTTGGCTCGCGCATCAGGCCGCGTACGACCTGTGGCAGGTG
GACCAGTACCGCAGCGAGCTGCGCGTGCGCTCGCTCCAGCCCGCGACCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References