Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1201542..1202341 Replicon chromosome
Accession NZ_CP072980
Organism Escherichia coli strain 2547

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KBW66_RS05735 Protein ID WP_000347251.1
Coordinates 1201542..1202006 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag KBW66_RS05740 Protein ID WP_001296435.1
Coordinates 1202006..1202341 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW66_RS05705 (1196543) 1196543..1196977 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KBW66_RS05710 (1196995) 1196995..1197873 - 879 WP_210796684.1 PTS N-acetylgalactosamine transporter subunit IID -
KBW66_RS05715 (1197863) 1197863..1198642 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KBW66_RS05720 (1198653) 1198653..1199126 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KBW66_RS05725 (1199149) 1199149..1200429 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
KBW66_RS05730 (1200678) 1200678..1201487 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KBW66_RS05735 (1201542) 1201542..1202006 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KBW66_RS05740 (1202006) 1202006..1202341 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KBW66_RS05745 (1202490) 1202490..1204061 - 1572 WP_001273763.1 galactarate dehydratase -
KBW66_RS05750 (1204436) 1204436..1205770 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KBW66_RS05755 (1205786) 1205786..1206556 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T199362 WP_000347251.1 NZ_CP072980:c1202006-1201542 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T199362 NZ_CP097329:2363320-2363583 [Pseudanabaena mucicola str. Chao 1806]
ATGAGGAACTTTGAATATGATGAAGATAAGAGTCAATCTAATTTTGCTAAACATGGAATTGACTTTGTTGAAGCTCAAAA
ACTATGGAACGATCCAAATTTGCTAGAAATTCCTAGTAGAATTCAAGATGAACCGCGATTTGTAGTTATTGGTAAAATAA
ATAGTAAACATTGGTCTGGAGTTATCACCTACCGCGATCAAAATATTCGGATTATATATGTCCGCCGATCACGCACACAA
GAGGTTGCCCTGTATGAACGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT199362 WP_001296435.1 NZ_CP072980:c1202341-1202006 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT199362 NZ_CP097329:2363573-2363806 [Pseudanabaena mucicola str. Chao 1806]
ATGAACGCTAAAGACTTTGATCAAAAATTCGACGATGGGGAAGAAGATATTATCGAAATGCTCGATCTATCTCAAGCTAA
ACGTCCCTTAGCTTCTCAAAAAAGGCTTAGCATAGAGTTACCTATTTGGATGATTGAGTTAATTAACCAAGAAGCCAAAC
GTTTAGGAGTAACACCACAAACAATTGTTCAAACATCTCTTGCTGAACATTTAGCAGTTAGTAACTTCAATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References