Detailed information of TA system TA199360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 388875..389477
• Replicon: chromosome
• Accession: NZ_CP072980
• Organism: Escherichia coli strain 2547

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: KBW66_RS01795
• Protein ID: WP_000897302.1
• Coordinates: 389166..389477 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KBW66_RS01790
• Protein ID: WP_000356397.1
• Coordinates: 388875..389165 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW66_RS01760 (384182)	384182..385111	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
KBW66_RS01765 (385293)	385293..385535	-	243	WP_001068514.1	CopG family transcriptional regulator	-
KBW66_RS01770 (385825)	385825..386673	+	849	WP_001038650.1	hypothetical protein	-
KBW66_RS26555 (386698)	386698..387438	+	741	WP_000608806.1	hypothetical protein	-
KBW66_RS01780 (387623)	387623..387841	-	219	WP_001251293.1	CopG family transcriptional regulator	-
KBW66_RS01785 (388238)	388238..388516	-	279	WP_001296612.1	hypothetical protein	-
KBW66_RS01790 (388875)	388875..389165	-	291	WP_000356397.1	NadS family protein	Antitoxin
KBW66_RS01795 (389166)	389166..389477	-	312	WP_000897302.1	hypothetical protein	Toxin
KBW66_RS01800 (389706)	389706..390614	+	909	WP_001385591.1	alpha/beta hydrolase	-
KBW66_RS01805 (390678)	390678..391619	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KBW66_RS01810 (391664)	391664..392101	-	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
KBW66_RS01815 (392098)	392098..392970	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KBW66_RS01820 (392964)	392964..393563	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T199360 WP_000897302.1 NZ_CP072980:c389477-389166 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T199360 NZ_CP097329:c1000904-1000617 [Pseudanabaena mucicola str. Chao 1806]
ATGAACAAAGTTACAACCTCTCTTTGGGATCCAGCAGAACATCTAGAAACGAAAGAAGATATGGCAGCTTATCTCGAAGC
CGCTCTTGAAGATGGCGATCCTAATTTAGTGGTAGCAGCATTAGGAGATATTGCTCGATCTAAAGGAATGACAAATGTTG
CGCGTGAAACAGGATTAGGTCGTGAAAGCCTTTATAAATCTTTGTCAATTGACGGTAACCCAGAATTTGCAACTGTTTTA
AGAGTTATACAGTCACTTGGCATCCGTTTACGAGCTACTCCTGTATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT199360 WP_000356397.1 NZ_CP072980:c389165-388875 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT199360 NZ_CP097329:c1001203-1000913 [Pseudanabaena mucicola str. Chao 1806]
ATGATTGAAATTCGGGAGACTAAAATCTATTCTCGGTGGTTCAAATCACTGCGCGATCGGGAAGCTAGGGCGCGTATCGA
CATTCGTATTCGGCGATTGTCTATGGGCAATCCTGGTGACGTAAAACCGATAGGAAAAGGTGTGTCAGAACTCCGTATTG
ACTATGGTGCAGGATACCGAGTGTATTTCATACAACGAGGTAATACGTTAATCATCTTGCTTGCAGGAGGCGATAAACGC
ACCCAAGATCGGGATATCAAAACAGCCTTAGACTTATCAGAAAACCTATAG

Structures

Toxin

• AlphaFold DB: S1P416