Detailed information of TA system TA199349

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 106936..107190
• Replicon: plasmid pEC2341_2
• Accession: NZ_CP072977
• Organism: Escherichia coli strain EC2341

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KBW65_RS23495
• Protein ID: WP_001312851.1
• Coordinates: 106936..107085 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	107129..107190 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW65_RS23465 (102183)	102183..103094	-	912	WP_000440183.1	carbamate kinase	-
KBW65_RS23470 (103105)	103105..104325	-	1221	WP_000410951.1	arginine deiminase	-
KBW65_RS23475 (105032)	105032..105646	+	615	Protein_113	VENN motif pre-toxin domain-containing protein	-
KBW65_RS23480 (105646)	105646..106092	-	447	Protein_114	plasmid replication initiator RepA	-
KBW65_RS23485 (106085)	106085..106159	-	75	WP_032336874.1	RepA leader peptide Tap	-
KBW65_RS23490 (106395)	106395..106652	-	258	WP_000083833.1	replication regulatory protein RepA	-
KBW65_RS23495 (106936)	106936..107085	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (107129)	107129..107190	+	62	NuclAT_0	-	Antitoxin
- (107129)	107129..107190	+	62	NuclAT_0	-	Antitoxin
- (107129)	107129..107190	+	62	NuclAT_0	-	Antitoxin
- (107129)	107129..107190	+	62	NuclAT_0	-	Antitoxin
KBW65_RS23500 (107329)	107329..107511	-	183	WP_000968309.1	hypothetical protein	-
KBW65_RS23505 (107612)	107612..108240	+	629	Protein_119	IS1-like element IS1A family transposase	-
KBW65_RS25105 (108307)	108307..108654	-	348	Protein_120	antirestriction protein	-
KBW65_RS23515 (109068)	109068..109838	-	771	WP_001006251.1	hypothetical protein	-
KBW65_RS23520 (109883)	109883..110401	-	519	WP_223349485.1	DUF1380 family protein	-
KBW65_RS23525 (110331)	110331..110552	-	222	WP_001104873.1	hypothetical protein	-
KBW65_RS23530 (110553)	110553..111236	-	684	WP_032181561.1	DNA methylase	-
KBW65_RS23535 (111313)	111313..111624	-	312	WP_012615291.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / oqxB / oqxA / tet(B) / catA1 / fosA3 / blaCTX-M-65 / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..144818	144818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T199349 WP_001312851.1 NZ_CP072977:c107085-106936 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T199349 NZ_CP097326:509043-509393 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT199349 NZ_CP072977:107129-107190 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin