Detailed information of TA system TA199336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3171780..3172507
• Replicon: chromosome
• Accession: NZ_CP072976
• Organism: Escherichia coli strain EC2341

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: KBW65_RS15275
• Protein ID: WP_000547564.1
• Coordinates: 3171780..3172091 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KBW65_RS15280
• Protein ID: WP_000126294.1
• Coordinates: 3172088..3172507 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW65_RS15245 (3166922)	3166922..3168631	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
KBW65_RS15250 (3168641)	3168641..3169183	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
KBW65_RS15255 (3169183)	3169183..3169950	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
KBW65_RS15260 (3169947)	3169947..3170357	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
KBW65_RS15265 (3170350)	3170350..3170820	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
KBW65_RS15270 (3170845)	3170845..3171606	+	762	WP_001026446.1	hypothetical protein	-
KBW65_RS15275 (3171780)	3171780..3172091	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
KBW65_RS15280 (3172088)	3172088..3172507	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
KBW65_RS15285 (3172621)	3172621..3174045	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
KBW65_RS15290 (3174054)	3174054..3175511	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
KBW65_RS15295 (3175771)	3175771..3176781	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
KBW65_RS15300 (3176930)	3176930..3177457	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T199336 WP_000547564.1 NZ_CP072976:3171780-3172091 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T199336 NZ_CP097320:3901912-3902196 [Candidatus Mycobacterium methanotrophicum]
GTGAGTTCTGCGCCTACGGACCCATACGAGCTCCGCATCACTCCTGAGGGACTCCGTCACCTGAACCGCCTCCCGGCGAA
GATCCGCGACGCAGCTCTGGCTGCGCTACATGGCCCCATCCGTGAAAACCCGCACCGGCTCGGCAGAGCGCTGGTCGGCG
AACTCGCCGGCCTTTTCTCGGCGCGACGAGGCGACTACCGGATCATCTACAGCATCGACGACACGGCCAAGATCGTCATC
GTGCACAGGATTGCGCATCGAGCGTCGGTGTACCGCCGACGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT199336 WP_000126294.1 NZ_CP072976:3172088-3172507 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT199336 NZ_CP097320:3901676-3901915 [Candidatus Mycobacterium methanotrophicum]
ATGCCCTTCTCCGAAGCGAAAGCCCACCTGTCCGAACTGGCCGAACGCGTCGAACTTGAGCATGCGCGCGTCCTCGTCAC
GCGCAACGGACGGCCATCATTCGTACTGGTGAGCCCCGACGACCTCGACGCACTCGAAGAGACGCTCGACATCCTGCGGG
ATCGCGAGCTTGTCGCCTCGCTGAAGCGGTCGCAAGAAGAGGCCGCTCAGGGTAGATCGCGGCCCTTGCCGTCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5