Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | prlF-yhaV (relBE)/YhaV-PrlF |
| Location | 2714933..2715732 | Replicon | chromosome |
| Accession | NZ_CP072976 | ||
| Organism | Escherichia coli strain EC2341 | ||
Toxin (Protein)
| Gene name | yhaV | Uniprot ID | S1NYM6 |
| Locus tag | KBW65_RS13060 | Protein ID | WP_000347251.1 |
| Coordinates | 2714933..2715397 (-) | Length | 155 a.a. |
Antitoxin (Protein)
| Gene name | prlF | Uniprot ID | S1EB98 |
| Locus tag | KBW65_RS13065 | Protein ID | WP_001307405.1 |
| Coordinates | 2715397..2715732 (-) | Length | 112 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KBW65_RS13030 (2709934) | 2709934..2710368 | - | 435 | WP_000948824.1 | PTS galactosamine/N-acetylgalactosamine transporter subunit IIA | - |
| KBW65_RS13035 (2710386) | 2710386..2711264 | - | 879 | WP_001295548.1 | PTS N-acetylgalactosamine transporter subunit IID | - |
| KBW65_RS13040 (2711254) | 2711254..2712033 | - | 780 | WP_000406214.1 | PTS N-acetylgalactosamine transporter subunit IIC | - |
| KBW65_RS13045 (2712044) | 2712044..2712517 | - | 474 | WP_001295547.1 | PTS N-acetylgalactosamine transporter subunit IIB | - |
| KBW65_RS13050 (2712540) | 2712540..2713820 | - | 1281 | WP_000681903.1 | tagatose-bisphosphate aldolase subunit KbaZ | - |
| KBW65_RS13055 (2714069) | 2714069..2714878 | + | 810 | WP_000072187.1 | aga operon transcriptional regulator AgaR | - |
| KBW65_RS13060 (2714933) | 2714933..2715397 | - | 465 | WP_000347251.1 | type II toxin-antitoxin system ribonuclease toxin YhaV | Toxin |
| KBW65_RS13065 (2715397) | 2715397..2715732 | - | 336 | WP_001307405.1 | type II toxin-antitoxin system antitoxin PrlF | Antitoxin |
| KBW65_RS13070 (2715881) | 2715881..2717452 | - | 1572 | WP_001273761.1 | galactarate dehydratase | - |
| KBW65_RS13075 (2717827) | 2717827..2719161 | + | 1335 | WP_000599652.1 | galactarate/glucarate/glycerate transporter GarP | - |
| KBW65_RS13080 (2719177) | 2719177..2719947 | + | 771 | WP_001058214.1 | 2-dehydro-3-deoxyglucarate aldolase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 155 a.a. Molecular weight: 17777.14 Da Isoelectric Point: 9.4947
>T199332 WP_000347251.1 NZ_CP072976:c2715397-2714933 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH
Download Length: 465 bp
>T199332 NZ_CP097320:c3452395-3451982 [Candidatus Mycobacterium methanotrophicum]
GTGATCTACCTGGACACCTCGGCCCTGGCCAAGCTGCTCATCGCCGAGCCGGAGACGCCCGAACTGCAGACCTGGCTCAC
CGCGCAAAGCGGGCAGGGCGAATACGCGGTGACCAGCGCGCTTGGCCGGGTCGAATTGATGAGAGTTGTTGCCCGATACG
GGGAACCGGGCCTGGCTGATCGCGCGCGCTATCTGCTCGACGGGCTCGACATCCTTCCGCTCGCCGAACCGGTGATCGCT
CTGGCGGAAACAATTGGCCCGCCCACTCTGTGCTCTCTCGATGCCATTCATCTCGCAGCCGCCGCCCATATCGAGCGGGA
ACTTGCCGTGTTCGTCACCTACGATCACCGGCTGTTGGACGGTTGCCGTGAGGTCGGCCTGGCATCCGCATCACCCGGTG
CCGCAGTTCCCTAA
GTGATCTACCTGGACACCTCGGCCCTGGCCAAGCTGCTCATCGCCGAGCCGGAGACGCCCGAACTGCAGACCTGGCTCAC
CGCGCAAAGCGGGCAGGGCGAATACGCGGTGACCAGCGCGCTTGGCCGGGTCGAATTGATGAGAGTTGTTGCCCGATACG
GGGAACCGGGCCTGGCTGATCGCGCGCGCTATCTGCTCGACGGGCTCGACATCCTTCCGCTCGCCGAACCGGTGATCGCT
CTGGCGGAAACAATTGGCCCGCCCACTCTGTGCTCTCTCGATGCCATTCATCTCGCAGCCGCCGCCCATATCGAGCGGGA
ACTTGCCGTGTTCGTCACCTACGATCACCGGCTGTTGGACGGTTGCCGTGAGGTCGGCCTGGCATCCGCATCACCCGGTG
CCGCAGTTCCCTAA
Antitoxin
Download Length: 112 a.a. Molecular weight: 12358.94 Da Isoelectric Point: 4.8616
>AT199332 WP_001307405.1 NZ_CP072976:c2715732-2715397 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE
Download Length: 336 bp
>AT199332 NZ_CP097320:c3452670-3452392 [Candidatus Mycobacterium methanotrophicum]
GTGGAGGCAATCGGAGTTCGCGAGTTGCGGCAGCACGCATCGCGATACCTCGCCCGGGTGCAAGCCGGCGAGGAGATCGG
CGTCACCAGCAACGGACGACTCGTAGCCCGACTCGTCCCCGTGCAGGCCCCGGCACGGTCTCGCGAAGCACTGATCGATG
CCGGTGCCCTGATTCCGGCGCGGCACCCGCAAAACCTTCTGGACGTCACGGCCGCACCCGCGCGGGACGGCAAGCGCACC
TTGTCCGATGTCCTCAACGAGATACGCAACGAGCAGTGA
GTGGAGGCAATCGGAGTTCGCGAGTTGCGGCAGCACGCATCGCGATACCTCGCCCGGGTGCAAGCCGGCGAGGAGATCGG
CGTCACCAGCAACGGACGACTCGTAGCCCGACTCGTCCCCGTGCAGGCCCCGGCACGGTCTCGCGAAGCACTGATCGATG
CCGGTGCCCTGATTCCGGCGCGGCACCCGCAAAACCTTCTGGACGTCACGGCCGCACCCGCGCGGGACGGCAAGCGCACC
TTGTCCGATGTCCTCAACGAGATACGCAACGAGCAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CJ20 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0XVC7 |