Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1189081..1189808 Replicon chromosome
Accession NZ_CP072961
Organism Enterobacter sp. MGH 14

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag IBG17_RS05505 Protein ID WP_022650547.1
Coordinates 1189497..1189808 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3V464
Locus tag IBG17_RS05500 Protein ID WP_022650546.1
Coordinates 1189081..1189500 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBG17_RS05485 1186153..1187274 - 1122 WP_003858868.1 YbdK family carboxylate-amine ligase -
IBG17_RS05490 1187380..1188270 + 891 WP_022650544.1 oxidoreductase -
IBG17_RS05495 1188323..1188757 - 435 WP_022650545.1 hypothetical protein -
IBG17_RS05500 1189081..1189500 - 420 WP_022650546.1 helix-turn-helix domain-containing protein Antitoxin
IBG17_RS05505 1189497..1189808 - 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
IBG17_RS26635 1190264..1190920 + 657 WP_022650548.1 hypothetical protein -
IBG17_RS05515 1191033..1191488 - 456 WP_022650549.1 hypothetical protein -
IBG17_RS05520 1191771..1192652 - 882 WP_022650550.1 LysR family transcriptional regulator -
IBG17_RS05525 1192758..1193537 + 780 WP_003858848.1 acetolactate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T199296 WP_022650547.1 NZ_CP072961:c1189808-1189497 [Enterobacter sp. MGH 14]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

>T199296 NZ_CP097287:30804-31100 [Pseudomonas viridiflava]
ATGAAAGAACCAATTTACGAATACGACCCAGCGGAATTACTCACCGATGCCGAATCTATCGCCATTTTCATGGCTGATGC
TTTTGAATCGGGCGATGCATCATTCATCGCGCAGGCGCTGAGCGTGGTAGCTCGCGCCAAAGGCATGACCGCAATTGCAA
AAGAGGCAGGCGTTACCCGCGCACACCTCTACCAACTGAAAAACGGCAACCCCACTTTGAAAACCATGATCGGGGTCATG
CGTGCGGTCGGTCTGGACATGACAGCAAAACAACACGTCGATGTAGCCCACGGCTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15447.51 Da        Isoelectric Point: 4.6051

>AT199296 WP_022650546.1 NZ_CP072961:c1189500-1189081 [Enterobacter sp. MGH 14]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT199296 NZ_CP097287:30505-30807 [Pseudomonas viridiflava]
ATGAAAACGATTATCCAAACCGCCACTTACATGACTTGGGAACGCAAACTGCGTGACAAGAAGGCCAAACTGATTATCGC
GGCGCGGGTGTTGCGAGTTGCCCACGGCTTGCTCGGTGACGTGCAGCCAGTTGGTCAAGGCGTCAGTGAGTTGCGAATTC
ATCATGGTCCCGGCTACCGGGTGTATTTCCAGCAGCGCGGCGATCAGCTGGTCCTATTGCTCTGCGGTGGGGATAAAAGC
AGTCAAGCGCGGGACATTGAGACCGCTAAAACTCTGGCAAGCCAGTGGAGTGAAGACGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3V464

References