Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 322744..323330 Replicon chromosome
Accession NZ_CP072949
Organism Klebsiella pneumoniae MGH 39

Toxin (Protein)


Gene name doc Uniprot ID A0A331ME34
Locus tag IBD60_RS01495 Protein ID WP_025861226.1
Coordinates 322962..323330 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag IBD60_RS01490 Protein ID WP_004174006.1
Coordinates 322744..322965 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBD60_RS01470 318901..319827 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
IBD60_RS01475 319824..321101 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
IBD60_RS01480 321098..321865 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
IBD60_RS01485 321867..322580 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
IBD60_RS01490 322744..322965 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IBD60_RS01495 322962..323330 + 369 WP_025861226.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IBD60_RS01500 323603..324919 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
IBD60_RS01505 325026..325913 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
IBD60_RS01510 325910..326755 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
IBD60_RS01515 326757..327827 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 319824..328564 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13551.98 Da        Isoelectric Point: 8.6410

>T199254 WP_025861226.1 NZ_CP072949:322962-323330 [Klebsiella pneumoniae MGH 39]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGIKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T199254 NZ_CP097255:c2955049-2954717 [Enterobacter bugandensis]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCAGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCGT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTAGTGGTTCCCGTCACCAGCGGCGGTAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCGGGGACAAAAACGATGGGCGTTATTCGCTGCGACCAGCCCAGAACTATT
GATATGGCTGCCCGAAACGGCATGCGGCTGGAACGCATACCTGATGCCGTTGTAAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTGAATTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT199254 WP_004174006.1 NZ_CP072949:322744-322965 [Klebsiella pneumoniae MGH 39]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT199254 NZ_CP097255:c2955307-2955050 [Enterobacter bugandensis]
ATGTATAAAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGTTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAACAATGGCTGCCTGATCATTGAACCCCAAAAACGGCCTCATTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCTGAAATGAGTGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331ME34

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References