Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 850158..850734 Replicon chromosome
Accession NZ_CP072935
Organism Corynebacterium genitalium ATCC 33030 strain CCUG 38989

Toxin (Protein)


Gene name graT Uniprot ID D7WDU2
Locus tag KBP53_RS04190 Protein ID WP_005290974.1
Coordinates 850158..850439 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag KBP53_RS04195 Protein ID WP_040424570.1
Coordinates 850474..850734 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBP53_RS04160 (KBP53_04150) 845818..846903 + 1086 WP_256001611.1 redox-regulated ATPase YchF -
KBP53_RS04165 (KBP53_04155) 846907..847296 - 390 WP_005291006.1 peptide chain release factor I -
KBP53_RS04170 (KBP53_04160) 847489..847839 - 351 WP_005291003.1 hypothetical protein -
KBP53_RS04175 (KBP53_04165) 847836..849257 - 1422 WP_005290998.1 mercury(II) reductase -
KBP53_RS04180 (KBP53_04170) 849356..849745 + 390 WP_005290994.1 heavy metal-responsive transcriptional regulator -
KBP53_RS04185 (KBP53_04175) 849792..850036 - 245 Protein_813 alkane 1-monooxygenase -
KBP53_RS04190 (KBP53_04180) 850158..850439 + 282 WP_005290974.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KBP53_RS04195 (KBP53_04185) 850474..850734 + 261 WP_040424570.1 HigA family addiction module antitoxin Antitoxin
KBP53_RS04200 (KBP53_04190) 850789..851232 - 444 WP_040424568.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
KBP53_RS04205 (KBP53_04195) 851543..852865 - 1323 WP_005290957.1 hypothetical protein -
KBP53_RS04210 (KBP53_04200) 852918..853064 - 147 WP_005290955.1 hypothetical protein -
KBP53_RS04215 (KBP53_04205) 853314..853436 + 123 Protein_819 addiction module antidote protein, HigA family -
KBP53_RS04220 (KBP53_04210) 853681..854661 + 981 WP_005290950.1 NAD(P)-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 847836..866952 19116


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-54)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11051.53 Da        Isoelectric Point: 9.8469

>T199234 WP_005290974.1 NZ_CP072935:850158-850439 [Corynebacterium genitalium ATCC 33030]
MIQSFADKDTERLWNRERVRSIDSRIHSVALRKLRQLGYAQTLDELRIPPGNRLEALKGDRRGQFSIRINDQWRICFRWS
AAGPEEVEIVDYH

Download         Length: 282 bp

>T199234 NZ_CP097248:4011175-4011277 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9730.29 Da        Isoelectric Point: 6.2179

>AT199234 WP_040424570.1 NZ_CP072935:850474-850734 [Corynebacterium genitalium ATCC 33030]
MEDFLKEMGITQHKLAVSIGVPPRRINEIVHGKRAVTADTALRLAKFFEMSPQFWLGLQTQYDLDVAEDKILAEIERIQP
VHAVSA

Download         Length: 261 bp

>AT199234 NZ_CP097248:c4011284-4011139 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F1ZH13

Antitoxin

Source ID Structure

References